Neuronal regulation of expression of hydrogen sulfide-producing enzyme cystathionine β-synthase in rat spinal cord astrocytes

Neuroscience Research
Ryo MiyamotoShigeo Ito

Abstract

Cystathionine β-synthase (CBS), expressed in astrocytes, generates a gaseous neuromodulator, hydrogen sulfide (H2S) in the central nervous system (CNS). However, little is known about the regulatory mechanisms of astrocytic CBS expression and activity. This study evaluated the influence of neurons on astrocytic CBS expression by employing multiple culture systems. Substantial CBS expression was observed in the intact neonatal rat spinal cord, while CBS content was markedly reduced in an astrocyte-enriched culture prepared from the neonatal spinal cord. Immunofluorescence analysis confirmed the localization of spinal cord CBS in astrocytes, but not in neurons. Although CBS expression was weak in the embryonic rat spinal cord, enzyme levels were time-dependently increased in a neuron/astrocyte mixed culture originating from embryonic spinal cord. The reduced CBS expression in isolated neonatal astrocytes was restored by co-culture with embryonic neurons. Together with the observed CBS expression levels, H2S production was relatively low in astrocytes cultured alone, but was considerably higher in astrocytes cultured with neurons. These results indicate that neurons are essential for maintaining the expression and H2S-producing ac...Continue Reading

References

Feb 1, 1985·The Journal of Cell Biology·M E Hatten
Jun 1, 1980·The Journal of Cell Biology·K D McCarthy, J de Vellis
Jan 1, 1995·Journal of Neural Transmission. General Section·B ReglandM Medhus
Jun 28, 2000·The Journal of Cell Biology·N RouachC Giaume
Feb 20, 2003·Trends in Neurosciences·Mark P Mattson, Thomas B Shea
Mar 27, 1964·Science·S H MUDDL LASTER
May 25, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yuka Kimura, Hideo Kimura
Jun 26, 2004·The Journal of Physiology·Tommaso Fellin, Giorgio Carmignoto
Jul 17, 2004·Journal of Neurochemistry·Matthew WhitemanPhilip K Moore
Dec 7, 2005·Spinal Cord·M Y LeeB J Kiratli
Sep 27, 2008·Neuron·Cendra AgulhonKen D McCarthy
Oct 16, 2008·Antioxidants & Redox Signaling·Norihiro ShibuyaHideo Kimura
Jul 28, 2009·Neurobiology of Aging·Moonhee LeePatrick L McGeer
Aug 8, 2009·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·F ValentinoV La Bella
Jan 18, 2011·Antioxidants & Redox Signaling·Sanjay K GargRuma Banerjee
Aug 1, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Michela CampoloSalvatore Cuzzocrea
Feb 5, 2014·Nitric Oxide : Biology and Chemistry·Hideo Kimura
Apr 30, 2014·EMBO Reports·Kexin ZhaoGuangdong Yang

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Citations

Aug 5, 2017·Antioxidants·Gethin J McBean
Oct 28, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Brennah MurphyPriyabrata Mukherjee
Mar 7, 2018·Oxidative Medicine and Cellular Longevity·Ulfuara ShefaJunyang Jung
Apr 1, 2021·Neurotoxicity Research·Angela T S WyseAndré Quincozes-Santos
Apr 6, 2021·Frontiers in Cell and Developmental Biology·Shizhen ZhaoHonggang Wang

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