Neuronal stimulation leading to upregulation of glutamate transporter-1 (GLT-1) in rat microglia in vitro

Neuroscience Letters
Kazuyuki NakajimaTadashi Kurihara

Abstract

As previously reported, activated microglia facilitate the expression of a glial cell-type glutamate transporter, glutamate transporter-1 (GLT-1; EAAT2), around injured motoneurons in axotomized rat facial nucleus. This phenomenon suggests that the motoneurons stimulate microglia to enhance the levels of GLT-1. In the present study, we investigated the effects of neuronal stimulus on the uptake of glutamate (Glu) by microglia and on the expression of GLT-1 protein in microglia in vitro. A 14C-Glu uptake experiment revealed that microglia enhance uptake of Glu by stimulation with neuronal conditioned medium (NCM). The NCM-stimulated uptake was significantly suppressed in the presence of dihydrokinate (a specific GLT-1 inhibitor), suggesting that GLT-1 is a major glutamate transporter for the uptake. Furthermore, immunoblotting analysis revealed that the amounts of GLT-1, but not another glial cell-type glutamate transporter glutamate-aspartate transporter (GLAST: EAAT1), increased significantly in microglia by treatment with NCM. Altogether, neuronal stimulus was found to promote the uptake of Glu in microglia, probably due to the increased levels of GLT-1.

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Citations

Dec 5, 2013·Clinical & Developmental Immunology·Brenda RocamondeJosé Miguel Soria
Mar 26, 2014·Journal of Molecular Neuroscience : MN·Kaifu KeMaohong Cao
Jun 5, 2015·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·Pengyue ZhangCe Xu
May 21, 2015·Brain Structure & Function·Joanna L ElsonIlse S Pienaar
Oct 19, 2016·Neurobiology of Disease·Carlo PeregoMaria-Grazia De Simoni
Jan 31, 2017·Journal of Neuroscience Research·Hongfei QiaoZhonghen Wu
May 23, 2018·The European Journal of Neuroscience·Ioanna SandvigAxel Sandvig
Jan 8, 2018·Reviews in the Neurosciences·Elena D OsipovaAlla B Salmina
Apr 30, 2011·Physiological Reviews·Helmut KettenmannAlexei Verkhratsky
Feb 25, 2020·Translational Stroke Research·Jialiang WeiGuohua Xi
Aug 28, 2021·Life Sciences·Dapinder KaurSimona Bungau
Aug 31, 2021·Cellular and Molecular Neurobiology·Bhakta Prasad Gaire

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