PMID: 9427522Jan 14, 1998Paper

Neuronal subtype-specific expression directed by the GABA(A) receptor delta subunit gene promoter/upstream region in transgenic mice and in cultured cells

Brain Research. Molecular Brain Research
B LüscherJ M Fritschy

Abstract

The promoter of the GABA(A) receptor delta subunit gene was analyzed in transgenic mice and in cultured cells to study sequences involved in neuronal subtype-specific gene expression. A 6.4-kb genomic fragment faithfully directed neuron-specific transcription of a lacZ reporter gene in the central nervous system. The transgene expression pattern in the cerebral cortex, hippocampal formation, thalamus, and brainstem was consistent with the regional and neuronal subtype-specific expression of the endogenous delta subunit protein in both developing and mature brain. In the cerebellum, however, the transgene was ectopically expressed in Purkinje cells and silent in granule cells, where the endogenous delta subunit is abundantly expressed. These mice provide a useful tool for investigating activity-dependent regulation of GABA(A) receptor expression under physiological and pathological conditions. Transfection studies using primary cortical neurons and astroglial cells revealed that the delta subunit gene promoter was selectively active in neurons even when truncated to a 267-bp core fragment. In conclusion, the delta subunit gene promoter/upstream region contains the information for neuronal subtype-specific expression in the entir...Continue Reading

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Citations

Mar 20, 2004·Pharmacology & Therapeutics·Janine L Steiger, Shelley J Russek
Aug 2, 2001·Journal of Neuroscience Methods·T Wells, D A Carter
Apr 2, 1999·Brain Research. Molecular Brain Research·W Mu, D R Burt
Nov 17, 2010·Molecular Psychiatry·B LuscherN Sahir
Oct 2, 2002·Journal of Neurochemistry·Karoline Fuchs, Nevenka Celepirovic
Jul 13, 2004·Pharmacology & Therapeutics·Bernhard Lüscher, Cheryl A Keller
Mar 15, 2000·Reviews in the Neurosciences·M Bader
Jul 5, 2011·The European Journal of Neuroscience·Venceslas DuveauJean-Marc Fritschy
Jul 11, 2013·The Journal of Biological Chemistry·Xia WuGong Chen

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