Neuronal transfection using particle-mediated gene transfer

Current Protocols in Neuroscience
D C Lo

Abstract

This unit describes the use of particle-mediated gene transfer (also known as biolistics) for the transfection of neuronal cell lines and brain slices. Like nuclear microinjection of DNA, biolistics results in the direct introduction of DNA into the nucleus; it is perhaps for this reason that biolistics works as well in mitotic cells as in postmitotic cells such as skeletal muscle, skin, liver, and neurons. The basic principle of biolistics is to accelerate micron-sized gold particles coated with DNA towards target cells or tissue. Cells penetrated by these particles have a high likelihood of being transfected by the DNA thus introduced. The motive force for particle acceleration can come from a variety of sources, the most widely used is described in this unit and is a supersonic shock wave generated by the rupture of a kapton membrane induced by high-pressure helium. Another option included in this unit is to propel the gold particles by gas jet entrainment.

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Apr 1, 1991·Journal of Neuroscience Methods·L StoppiniD Muller
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R S WilliamsJ C Sanford
Feb 1, 1990·Current Genetics·D ArmaleoS A Johnston
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N S YangD McCabe
Aug 23, 1990·Nature·S A Johnston
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·D ArnoldN Heintz
Feb 11, 1994·Science·M ChalfieD C Prasher
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·N T SherwoodD C Lo
Jan 1, 1992·Current Biology : CB·L T Pecorino, D C Lo

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Citations

May 24, 2013·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Barbara CalaminiLinda S Kaltenbach

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