Neurons from fetal rat brains contain functional luteinizing hormone/chorionic gonadotropin receptors

Biology of Reproduction
A A AL-HaderC V Rao

Abstract

Adult and neonatal rat brains contain functional LH/hCG receptors. These findings have led us to hypothesize that the fetal rat brain may also contain these receptors. To test this hypothesis, we isolated neurons from 19-day-old fetal rat brains and cultured them in chemically defined serum-free medium. The reverse transcription polymerase chain reaction amplified an expected 256-base pair size LH/hCG receptor fragment that could hybridize with a full-length LH/hCG receptor cDNA in Southern blotting. Northern blotting demonstrated that neurons contained a major 2.6 kilobase (kb) and a minor 4.3 kb transcript. Immunocytochemistry demonstrated that the neurons contained LH/hCG receptor immunostaining. Western immunoblotting showed that neurons contained an 80-kDa receptor protein that increased to a maximal level on Day 3 of culture and then gradually decreased until the 9th day of culture. Culturing neurons for 3 days in the presence of highly purified hCG resulted in a dose-dependent increase in the outgrowth of neurite processes and total cellular protein and a decrease in DNA fragmentation as compared to values in the corresponding controls. At the maximally effective hCG concentration, the number of neurite-bearing cells was...Continue Reading

Citations

Dec 31, 2002·Neuroscience Letters·Satish C RaoDavid S K Magnuson
Mar 16, 2000·Journal of Neuroendocrinology·R L BowenR L Atkinson
Jan 26, 2008·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·A JankowskaJ B Warchol
Feb 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Eun-Jin YangDarcy B Kelley
Sep 19, 2001·Mayo Clinic Proceedings·R A ShortN R Graff-Radford
Oct 29, 2008·Brain Research Bulletin·Longxuan LiWilliam Z Suo
Jul 31, 2007·Neurobiology of Disease·Lani K ClintonFrank M LaFerla
Jul 15, 2015·Hormones and Behavior·Jeffrey A BlairGemma Casadesus
Nov 4, 2004·BJOG : an International Journal of Obstetrics and Gynaecology·S D KeayH S Randeva
Jun 29, 2011·Clinical and Translational Science·Rosemeire M Kanashiro-TakeuchiJoshua M Hare
Apr 3, 2008·Biochimica Et Biophysica Acta·Andrea C WilsonCraig S Atwood
Oct 9, 2012·Neuron·Anthony N van den Pol
Feb 15, 2019·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M JahanshahiG Bahlakeh
Oct 23, 2002·Journal of Neuroscience Research·Richard L BowenCraig S Atwood
Nov 10, 2021·Reproduction in Domestic Animals = Zuchthygiene·Yongfu LaPing Yan

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