Neuropeptide Y inhibits depolarization-stimulated catecholamine synthesis in rat pheochromocytoma cells

European Journal of Pharmacology
L A McCullough, T C Westfall

Abstract

In PC12 rat pheochromocytoma cells differentiated with nerve growth factor (NGF), neuropeptide Y inhibited depolarization-stimulated catecholamine synthesis as determined by in situ measurement of 3,4-dihydroxyphenylalanine (DOPA) production in the presence of the decarboxylase inhibitor m-hydroxybenzylhydrazine (NSD-1015). The inhibition by neuropeptide Y was concentration-dependent and was prevented by pretreatment with pertussis toxin, suggesting the involvement of a GTP-binding protein of the Gi or Go subtype. The neuropeptide Y analog [Leu31,Pro34]neuropeptide Y also caused inhibition of DOPA production, but was less potent than neuropeptide Y itself, while peptide YY and neuropeptide Y-(13-36) had no significant effect. This pattern is most consistent with the involvement of the neuropeptide Y Y3 receptor subtype. In PC12 cells differentiated with dexamethasone, neuropeptide Y also caused a concentration-dependent inhibition of DOPA production, while peptide YY was again without effect. Neuropeptide Y had no effect on DOPA production in undifferentiated PC12 cells. These results indicate that neuropeptide Y can modulate catecholamine synthesis in addition to its modulatory effects on catecholamine release.

References

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Citations

Jun 2, 2005·Regulatory Peptides·Adepero Shola AdewaleThomas C Westfall
Nov 5, 1998·Regulatory Peptides·C C Lee, R J Miller
Nov 5, 1998·Regulatory Peptides·L A McCulloughT C Westfall
Apr 5, 2001·Peptides·D Renshaw, J P Hinson
Jun 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Cláudia CavadasEric Grouzmann
Nov 9, 2011·The Biochemical Journal·María T Marcos-AlmarazAlberto Pascual
Jul 1, 2006·Journal of Cardiovascular Pharmacology·Thomas C WestfallChun-Lian Yang
Apr 12, 2000·Journal of Peptide Science : an Official Publication of the European Peptide Society·C Cabrele, A G Beck-Sickinger
Nov 15, 1997·The American Journal of Physiology·X ChenT C Westfall
Jun 5, 1998·The American Journal of Physiology·L A McCulloughT C Westfall
Dec 13, 2005·Experimental Biology and Medicine·Jill L SmithJonathan S Fisher
Jun 18, 2004·General and Comparative Endocrinology·Anna CapaldoLorenzo Varano

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