PMID: 8945904Nov 1, 1996Paper

Neuropeptide Y reduces macromolecule permeability of coronary endothelial monolayers

The American Journal of Physiology
T NollH M Piper

Abstract

The effect of neuropeptide Y (NPY) on cellular adenosine 3',5'-cyclic monophosphate (cAMP) contents and macromolecule permeability was studied in cultured monolayers of microvascular coronary endothelial cells from rat. Macromolecule permeability was continuously determined as passage of albumin across the monolayers. NPY (10(-10)-10(-7) M) decreased albumin flux and cellular cAMP content in a dose-dependent manner, with a half-maximal effect on albumin flux at 1.4 x 10(-9) M and on cAMP contents at 0.7 x 10(-9) M. A maximum effect of NPY was observed at 10(-7) M, decreasing albumin flux by 71 +/- 8% and cellular cAMP contents by 80 +/- 9% (mean +/- SD, n = 6, P < 0.05) compared with control. The effect of NPY on albumin flux was not altered in the presence of 10(-5) M indomethacin (an inhibitor of cyclooxygenase) and 10(-5) M NG-nitro-L-arginine (an inhibitor of nitric oxide synthase). NPY (10(-7) M) also antagonized the increase of albumin flux and cAMP content induced by 10(-6) M isoproterenol. Pretreatment of endothelial monolayers with pertussis toxin (1 microgram/ml for 2 h) abolished the effect of NPY on albumin flux and cAMP contents. This study shows that NPY can modulate macromolecule permeability of endothelial monol...Continue Reading

Citations

Oct 25, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Yong-Shan NanNaohisa Ishikawa
Oct 12, 2004·American Journal of Physiology. Cell Physiology·K BindewaldT Noll
Feb 21, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Nakako KurimotoNaohisa Ishikawa

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