Neuropeptide Y, Y1, Y2 and Y4 receptors mediate Y agonist responses in isolated human colon mucosa

British Journal of Pharmacology
Helen M Cox, Iain R Tough

Abstract

1. The aim of this study was to provide a pharmacological characterization of the Y receptor types responsible for neuropeptide Y (NPY), peptide YY (PYY) and pancreatic polypeptide (PP) effects upon electrogenic ion transport in isolated human colonic mucosa. 2. Preparations of descending colon were voltage-clamped at 0 mV in Ussing chambers and changes in short-circuit current (I(sc)) continuously recorded. Basolateral PYY, NPY, human PP (hPP), PYY(3 - 36), [Leu(31), Pro(34)]PYY (Pro(34)PYY) and [Leu(31), Pro(34)]-NPY (Pro(34)NPY) all reduced basal I(sc) in untreated colon. Of all the Y agonists tested PYY(3 - 36) responses were most sensitive to tetrodotoxin (TTX) pretreatment, indicating that Y(2)-receptors are located on intrinsic neurones as well as epithelia in this tissue. 3. The EC(50) values for Pro(34)PYY, PYY(3 - 36) and hPP were 9.7 nM (4.0 - 23.5), 11.4 nM (7.6 - 17.0) and 14.5 nM (10.2 - 20.5) and response curves exhibited similar efficacies. The novel Y(5) agonist [Ala(31), Aib(32)]-NPY had no effect at 100 nM. 4. Y(1) receptor antagonists, BIBP3226 and BIBO3304 both increased basal I(sc) levels per se and inhibited subsequent PYY and Pro(34)PYY but not hPP or PYY(3 - 36) responses. The Y(2) antagonist, BIIE0246 ...Continue Reading

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Citations

Oct 26, 2010·EMBO Molecular Medicine·Shaun P Brothers, Claes Wahlestedt
Nov 1, 2005·Breast Cancer Research and Treatment·R J SantenR X Song
Jan 24, 2014·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S PatelH M Cox
Mar 24, 2015·Progress in Retinal and Eye Research·Ana Santos-CarvalhoCláudia Cavadas
Dec 30, 2006·Peptides·Helen M Cox
Jul 30, 2014·Angewandte Chemie·Veronika MädeAnnette G Beck-Sickinger
Aug 24, 2018·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Iain R ToughHelen M Cox
Mar 29, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Xavier MontetLee Josephson
May 21, 2014·Cell and Tissue Research·Hyun-Jung ChoJohn B Furness
Sep 3, 2013·British Journal of Pharmacology·S JoshiH M Cox
Jun 30, 2006·The Journal of Pharmacology and Experimental Therapeutics·Iain R ToughHelen M Cox
Dec 4, 2004·Molecular Pharmacology·Nicholas D HollidayHelen M Cox
Aug 16, 2017·World Journal of Gastroenterology : WJG·Magdy El-SalhyJan Gunnar Hatlebakk
Dec 16, 2010·Current Opinion in Endocrinology, Diabetes, and Obesity·Amy D NguyenAmanda Sainsbury
Jun 6, 2003·British Journal of Pharmacology·Nicholas D Holliday, Helen M Cox
Nov 16, 2016·Cell and Tissue Research·Patricia MartinsJohn B Furness
Mar 11, 2003·Experimental Biology and Medicine·Magnus M BerglundDonald R Gehlert
Jul 19, 2014·European Journal of Pharmacology·Shu-ichi KojimaNaohiko Anzai
Jun 26, 2021·World Journal of Gastroenterology : WJG·Magdy El-SalhySutep Gonlachanvit
Dec 5, 2006·Autonomic Neuroscience : Basic & Clinical·Helen M Cox

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