NeuroPG: open source software for optical pattern generation and data acquisition

Frontiers in Neuroengineering
Benjamin W AvantsJacob T Robinson

Abstract

Patterned illumination using a digital micromirror device (DMD) is a powerful tool for optogenetics. Compared to a scanning laser, DMDs are inexpensive and can easily create complex illumination patterns. Combining these complex spatiotemporal illumination patterns with optogenetics allows DMD-equipped microscopes to probe neural circuits by selectively manipulating the activity of many individual cells or many subcellular regions at the same time. To use DMDs to study neural activity, scientists must develop specialized software to coordinate optical stimulation patterns with the acquisition of electrophysiological and fluorescence data. To meet this growing need we have developed an open source optical pattern generation software for neuroscience-NeuroPG-that combines, DMD control, sample visualization, and data acquisition in one application. Built on a MATLAB platform, NeuroPG can also process, analyze, and visualize data. The software is designed specifically for the Mightex Polygon400; however, as an open source package, NeuroPG can be modified to incorporate any data acquisition, imaging, or illumination equipment that is compatible with MATLAB's Data Acquisition and Image Acquisition toolboxes.

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Citations

May 6, 2016·Frontiers in Neuroinformatics·Benjamin D EvansKonstantin Nikolic
Feb 20, 2021·Journal of Neural Engineering·Le Cai, Philipp Gutruf
Jul 22, 2021·Nature Communications·Mizuki HondaYasuyuki Ohkawa
Sep 8, 2021·Frontiers in Cell and Developmental Biology·Yu TangAlexander A Chubykin

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Methods Mentioned

BETA
transgenic
fluorescence microscopy

Software Mentioned

NueroPG
Linux
CameraWindow
SmartGrid
MATLAB
OSX
MATLAB Image Acquisition Toolbox
Windows
pClamp
HeatMap

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