Neurophysiological defects and neuronal gene deregulation in Drosophila mir-124 mutants.

PLoS Genetics
Kailiang SunEric C Lai

Abstract

miR-124 is conserved in sequence and neuronal expression across the animal kingdom and is predicted to have hundreds of mRNA targets. Diverse defects in neural development and function were reported from miR-124 antisense studies in vertebrates, but a nematode knockout of mir-124 surprisingly lacked detectable phenotypes. To provide genetic insight from Drosophila, we deleted its single mir-124 locus and found that it is dispensable for gross aspects of neural specification and differentiation. On the other hand, we detected a variety of mutant phenotypes that were rescuable by a mir-124 genomic transgene, including short lifespan, increased dendrite variation, impaired larval locomotion, and aberrant synaptic release at the NMJ. These phenotypes reflect extensive requirements of miR-124 even under optimal culture conditions. Comparison of the transcriptomes of cells from wild-type and mir-124 mutant animals, purified on the basis of mir-124 promoter activity, revealed broad upregulation of direct miR-124 targets. However, in contrast to the proposed mutual exclusion model for miR-124 function, its functional targets were relatively highly expressed in miR-124-expressing cells and were not enriched in genes annotated with epide...Continue Reading

References

May 15, 2002·Current Biology : CB·Mariana Lagos-QuintanaThomas Tuschl
Feb 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Wei J Gong, Kent G Golic
Feb 21, 2003·Current Biology : CB·Josh DubnauTim Tully
Dec 24, 2003·PLoS Biology·Alexander StarkStephen M Cohen
Mar 12, 2004·Developmental Biology·Nikolaus Rajewsky, Nicholas D Socci
Mar 12, 2004·Genes & Development·John G Doench, Phillip A Sharp
Mar 30, 2004·Neuron·Brian D McCabeA Pejmun Haghighi
May 15, 2004·Nature Reviews. Genetics·David P Bartel, Chang-Zheng Chen
Feb 22, 2005·PLoS Biology·Julius BrenneckeStephen M Cohen
Mar 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Harlan RobinsRichard W Padgett
May 28, 2005·Science·Erno WienholdsRonald H A Plasterk
Dec 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·A Aziz AboobakerEric C Lai
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Pranidhi SoodNikolaus Rajewsky
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·André P GerberDaniel Herschlag
Mar 9, 2007·Genes & Development·Xinwei CaoFred H Gage
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·D MoazedS P Gygi
Jul 7, 2007·Molecular Cell·Andrew GrimsonDavid P Bartel
Jul 25, 2007·Genome Biology·Pavel TomancakGerald M Rubin
Nov 9, 2007·Genome Research·Alexander StarkManolis Kellis
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Fedor V KarginovGregory J Hannon
Feb 19, 2008·Nature·Jonathan B JohnnidisFernando D Camargo
Jun 10, 2008·Current Opinion in Hematology·Louis M Pelus
Jul 16, 2008·Experimental Cell Research·Jenn-Yah YuDavid L Turner
Aug 1, 2008·Nature·Daehyun BaekDavid P Bartel
Aug 1, 2008·Nature·Matthias SelbachNikolaus Rajewsky
Aug 23, 2008·Cell Cycle·Peter Smibert, Eric C Lai

❮ Previous
Next ❯

Citations

Aug 30, 2014·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Chao WangLiudi Yuan
Feb 5, 2013·Current Opinion in Cell Biology·Alfred X SunAndrew S Yoo
Dec 5, 2015·Frontiers in Neurology·Gregory M DavisRoger Pocock
Jul 19, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Eyal Mor, Noam Shomron
Oct 30, 2012·Insect Biochemistry and Molecular Biology·Sassan Asgari
Aug 14, 2012·Neuron·Elizabeth McNeill, David Van Vactor
Apr 22, 2015·Neural Development·Dana A BurowMichael D Cleary
Jul 24, 2013·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Malin Åkerblom, Johan Jakobsson
Feb 6, 2017·Genome Research·Natalia PinzónHervé Seitz
Feb 7, 2019·Frontiers in Genetics·Zoe Butti, Shunmoogum A Patten
Jun 12, 2019·International Journal of Molecular Sciences·Serena MaggioMichele Guescini
Mar 7, 2019·Scientific Reports·Takashi KozukaTakahisa Furukawa
Mar 29, 2020·Briefings in Functional Genomics·Saeed SoleimaniSadegh Azimzadeh Jamalkandi
Apr 25, 2017·Genes, Chromosomes & Cancer·Yulia Margolin-MillerSmadar Avigad
Nov 16, 2016·Journal of Neurogenetics·Alfredo GhezziNigel S Atkinson
Nov 7, 2019·G3 : Genes - Genomes - Genetics·Nathan C DonelsonLeslie C Griffith

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
fluorescence activated cell sorting
transfection
genetic stress
PCR
in vitro transcription
Flow Cytometry

Software Mentioned

TargetScan
GCRMA
miREDUCE
SPSS
Excel
miRanda
Multiz
Deadpan
MARCM
Leica

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.