Neuropilin-1 is associated with clinicopathology of gastric cancer and contributes to cell proliferation and migration as multifunctional co-receptors

Journal of Experimental & Clinical Cancer Research : CR
Linhao LiXueying Sun

Abstract

Neuropilin-1 (NRP-1) is a transmembrane glycoprotein participating in the growth and metastasis of cancer cells as multifunctional co-receptors by interacting with the signaling pathways. However, its role in gastric cancer has not yet been clarified. This study aims to investigate whether NRP-1 expression is associated with the clinicopathology of gastric cancer, and involved in the growth and metastasis of gastric cancer cells. NRP-1 expression in clinical gastric cancer specimens was examined by immunohistochemistry and its association with clinicopathology analyzed. The expression of NRP-1 in a panel of human gastric cancer cells was examined by real-time RT-PCR and immunoblotting. Stable transfectants depleted of NRP-1, termed MGC-803-NRP(low), were generated from MGC-803 cells. Cell proliferation was analyzed by the Cell Counting Kit-8 and Bromodeoxyuridine incorporation assays, and migrating ability analyzed by migration assays. The xenograft model was used to assess the effects of NRP-1 depletion on tumorigenesis, growth, metastasis and therapeutic potentials. The role of NRP-1 as co-receptors in the signaling pathways stimulated by ligands was examined. The key molecules involved in cell proliferation, migration and re...Continue Reading

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Citations

Jun 16, 2017·Liver International : Official Journal of the International Association for the Study of the Liver·Huaqiang ZhuJun Lu
Mar 5, 2018·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Nyanbol KuolKulmira Nurgali
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Apr 10, 2021·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Muhammad Tarek Abdel GhafarRasha A Elkholy

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Methods Mentioned

BETA
transfection
flow cytometry

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