Neuropilin-2 interacts with VEGFR-2 and VEGFR-3 and promotes human endothelial cell survival and migration

Blood
Benoit FavierFrançoise Bono

Abstract

Neuropilin 2 (NRP2) is a receptor for the vascular endothelial growth factor (VEGF) and the semaphorin (SEMA) families, 2 unrelated ligand families involved in angiogenesis and neuronal guidance. NRP2 specifically binds VEGF-A and VEGF-C, although the biological relevance of these interactions in human endothelial cells is poorly understood. In this study, we show that both VEGF-A and VEGF-C induce the interaction of NRP2 with VEGFR-2. This interaction correlated with an enhancement of the VEGFR-2 phosphorylation threshold. Overexpression of NRP2 in primary human endothelial cells promoted cell survival induced by VEGF-A and VEGF-C. In contrast, SEMA3F, another ligand for NRP2, was able to inhibit human endothelial cell survival and migration induced by VEGF-A and VEGF-C. Moreover, a siRNA targeting specifically NRP2 was a potent inhibitor of human endothelial cell migration induced by VEGF-A and VEGF-C. Thus, our data indicate that NRP2 acts as a coreceptor that enhances human endothelial cell biological responses induced by VEGF-A and VEGF-C.

References

Apr 22, 1994·Science·P C BrooksD A Cheresh
Oct 1, 1995·The Journal of Clinical Investigation·P C BrooksD A Cheresh
Aug 22, 1997·Cell·A L KolodkinD D Ginty
Jun 8, 2000·The Journal of Biological Chemistry·G FuhA M de Vos
Aug 10, 2000·Journal of Neurobiology·F NakamuraS M Strittmatter
Dec 2, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H Q MiaoM Klagsbrun
Mar 30, 2001·The Journal of Biological Chemistry·Z Gluzman-PoltorakG Neufeld
Oct 26, 2001·Mechanisms of Development·Y HerzogG Neufeld
Feb 14, 2002·Trends in Cardiovascular Medicine·Gera NeufeldYael Herzog
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Seiji TakashimaMasatsugu Hori
Jul 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Percy LeeMichael Klagsbrun
Mar 5, 2003·Advances in Experimental Medicine and Biology·Gera NeufeldYael Herzog
Jun 5, 2003·Nature Medicine·Napoleone FerraraJennifer LeCouter
Feb 12, 2004·Cancer Research·Ofra KesslerGera Neufeld
Oct 12, 2004·Biochemical and Biophysical Research Communications·Antoine AlamJean-Marc Herbert
Oct 27, 2004·Molecular Medicine·Jikui ShenPeter A Campochiaro
Nov 3, 2004·The Journal of Clinical Investigation·Diane R BielenbergMichael Klagsbrun
Mar 23, 2005·Current Opinion in Cell Biology·Daniela HoellerIvan Dikic

❮ Previous
Next ❯

Citations

Mar 12, 2013·Cellular and Molecular Life Sciences : CMLS·Ina M Wittko-SchneiderKarl H Plate
Feb 13, 2009·Angiogenesis·Susana F Rocha, Ralf H Adams
Mar 7, 2009·Angiogenesis·Guido SeriniFederico Bussolino
Sep 5, 2007·Cancer Metastasis Reviews·Diane R Bielenberg, Michael Klagsbrun
Oct 30, 2012·Journal of Computer-aided Molecular Design·Eunkyoung JungDong Hyun Jung
Dec 14, 2011·Cell Research·Atsuko SakuraiJ Silvio Gutkind
Sep 17, 2009·Immunology and Cell Biology·Nicola F LintonChandrakant Tayade
Jun 27, 2008·Nature Reviews. Cancer·Gera Neufeld, Ofra Kessler
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Eva-Maria HedlundYihai Cao
Jan 10, 2008·Journal of the National Cancer Institute·Michael J GrayLee M Ellis
Jul 5, 2012·Cold Spring Harbor Perspectives in Medicine·Sina Koch, Lena Claesson-Welsh
Sep 26, 2008·American Journal of Physiology. Renal Physiology·Ricardo SabanRobert E Hurst
Jan 1, 2009·Physiological Genomics·Eugenia C Pacheco-PinedoTejvir S Khurana
Oct 20, 2007·The Journal of Clinical Investigation·Carrie J ShawberJan Kitajewski
Aug 7, 2012·Genes & Cancer·Ailsa Christiansen, Michael Detmar
Dec 11, 2007·Blood·Eric SulpiceTatyana Merkulova-Rainon
Jul 16, 2010·Blood·Karlien HermansPeter Carmeliet
Nov 9, 2011·BMC Physiology·Marcia R SabanRicardo Saban
Aug 12, 2010·Journal of Angiogenesis Research·Steven P WilliamsSteven A Stacker
Nov 17, 2011·PloS One·Phillip YenAleksander S Popel
Jun 3, 2011·The Oncologist·Alicia F C OkinesDavid Cunningham
Jan 25, 2013·International Journal of Molecular Sciences·Jui-Chieh ChenJen-Liang Su
Dec 18, 2013·Cytokine & Growth Factor Reviews·Prakash VempatiFeilim Mac Gabhann
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Irma AiroldiVito Pistoia
Nov 2, 2013·Pharmacology & Therapeutics·Orion P KeiferNicholas M Boulis
Aug 5, 2014·Microvascular Research·Matthew T MigliozziDiane R Bielenberg
Feb 4, 2010·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·Angelika Zabel-du BoisPeter E Huber
Feb 14, 2013·The Journal of Biological Chemistry·Nicole C HarrisMarc G Achen
Mar 12, 2013·Cellular and Molecular Life Sciences : CMLS·Sophie ChauvetFanny Mann
Jan 13, 2010·The Journal of Cell Biology·Yunling XuAnil Bagri
Jan 9, 2016·Cancer Letters·Darci M FinkMichael A Hollingsworth
Jan 16, 2013·Seminars in Cell & Developmental Biology·Claudio Raimondi, Christiana Ruhrberg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.