Neuroprotection and immunomodulation by dimethyl fumarate and a heterologous fibrin biopolymer after ventral root avulsion and reimplantation

The Journal of Venomous Animals and Toxins Including Tropical Diseases
Paula Regina Gelinski KempeAlexandre Leite Rodrigues de Oliveira

Abstract

Ventral root avulsion (VRA) is an experimental approach in which there is an abrupt separation of the motor roots from the surface of the spinal cord. As a result, most of the axotomized motoneurons degenerate by the second week after injury, and the significant loss of synapses and increased glial reaction triggers a chronic inflammatory state. Pharmacological treatment associated with root reimplantation is thought to overcome the degenerative effects of VRA. Therefore, treatment with dimethyl fumarate (DMF), a drug with neuroprotective and immunomodulatory effects, in combination with a heterologous fibrin sealant/biopolymer (FS), a biological glue, may improve the regenerative response. Adult female Lewis rats were subjected to VRA of L4-L6 roots followed by reimplantation and daily treatment with DMF for four weeks. Survival times were evaluated 1, 4 or 12 weeks after surgery. Neuronal survival assessed by Nissl staining, glial reactivity (anti-GFAP for astrocytes and anti-Iba-1 for microglia) and synapse preservation (anti-VGLUT1 for glutamatergic inputs and anti-GAD65 for GABAergic inputs) evaluated by immunofluorescence, gene expression (pro- and anti-inflammatory molecules) and motor function recovery were measured. Tr...Continue Reading

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Citations

Feb 11, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bruna Botteon Della ColettaRogério Leone Buchaim
Sep 1, 2020·Brain Research Bulletin·Nahanna Zimmermann Menezes CarvalhoAlexandre Leite Rodrigues Oliveira

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Datasets Mentioned

BETA
BR1020140114360

Methods Mentioned

BETA
dissection
electrophoresis
PCRs
PCR

Software Mentioned

GraphPad Prism
Catwalk
MaxPro
ImageJ

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