Neuroprotection by biodegradable PAMAM ester (e-PAM-R)-mediated HMGB1 siRNA delivery in primary cortical cultures and in the postischemic brain

Journal of Controlled Release : Official Journal of the Controlled Release Society
Il-Doo KimJa-Kyeong Lee

Abstract

Although RNA interference (RNAi)-mediated gene silencing provides a powerful strategy for modulating specific gene functions, difficulties associated with siRNA delivery have impeded the development of efficient therapeutic applications. In particular, the efficacy of siRNA delivery into neurons has been limited by extremely low transfection efficiencies. e-PAM-R is a biodegradable arginine ester of PAMAM dendrimer, which is readily degradable under physiological conditions (pH 7.4, 37 degrees C). In the present study, we investigated the efficiency of siRNA delivery by e-PAM-R in primary cortical cultures and in rat brain. e-PAM-R/siRNA complexes showed high transfection efficiencies and low cytotoxicities in primary cortical cultures. Localization of fluorescence-tagged siRNA revealed that siRNA was delivered not only into the nucleus and cytoplasm, but also along the processes of the neuron. e-PAM-R/siRNA complex-mediated target gene reduction was observed in over 40% of cells and it was persistent for over 48 h. The potential use of e-PAM-R was demonstrated by gene knockdown after transfecting High mobility group box-1 (HMGB1, a novel cytokine-like molecule) siRNA into H(2)O(2)- or NMDA-treated primary cortical cultures. In...Continue Reading

References

Jul 10, 1999·Science·H WangK J Tracey
Oct 3, 2002·Nature Reviews. Genetics·Michael T McManus, Phillip A Sharp
Apr 3, 2004·Nature Reviews. Drug Discovery·Yair Dorsett, Thomas Tuschl
May 19, 2004·Journal of Neurochemistry·Jung-Bin KimJa-Kyeong Lee
Sep 29, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Joon Sig ChoiJong-Sang Park
Feb 16, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Malavosklish BikramSung Wan Kim
Jun 16, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jung-Bin KimJa-Kyeong Lee
Jul 18, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jung-Bin KimJa-Kyeong Lee
Nov 17, 2006·Nature Chemical Biology·David BumcrotDinah W Y Sah
Mar 14, 2007·The Journal of Gene Medicine·A L C CardosoM C Pedroso de Lima
Apr 17, 2007·Advanced Drug Delivery Reviews·William M Pardridge
Jun 26, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dana J GaryYou-Yeon Won
Jul 6, 2007·Nature Clinical Practice. Neurology·Pedro Gonzalez-Alegre, Henry L Paulson
Nov 3, 2007·Journal of Neuroscience Research·Jung-Bin KimJa-Kyeong Lee
Nov 6, 2007·Immunological Reviews·Marco E Bianchi, Angelo A Manfredi
Nov 15, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jianhua QiuMichael A Moskowitz
Jan 19, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jordy LutenWim E Hennink
Aug 23, 2008·International Journal of Pharmaceutics·Hye Yeong NamJong-Sang Park
Sep 18, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·A L C CardosoC Culmsee
Feb 10, 2009·Biomaterials·Fenghua MengZhiyuan Zhong

❮ Previous
Next ❯

Citations

Jan 13, 2011·Pharmaceutical Research·Francisco C Pérez-MartínezValentín Ceña
Jan 12, 2013·Pharmaceutical Research·Taiyoun RhimMinhyung Lee
Jun 26, 2013·Pharmaceutical Research·Huile GaoXinguo Jiang
Oct 19, 2012·ACS Chemical Neuroscience·A M O'MahonyC M O'Driscoll
Jan 19, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Il-Doo KimJa-Kyeong Lee
Nov 10, 2013·International Journal of Nanomedicine·Bindu BalakrishnanSujatha Kannan
Apr 17, 2013·International Journal of Nanomedicine·Songhie AnJong-Sang Park
Nov 3, 2010·Nanomedicine·Inmaculada PosadasValentín Ceña
Mar 20, 2013·Cellular and Molecular Life Sciences : CMLS·Lisa GherardiniTommaso Pizzorusso
Aug 21, 2012·Therapeutic Delivery·Mariacristina GagliardiAngelo Bifone
Dec 1, 2010·Therapeutic Delivery·Olivia M MerkelThomas Kissel
Nov 30, 2013·TheScientificWorldJournal·Jiangyu WuZiying He
Jan 25, 2013·Pharmaceutical Research·Fabiana Testa Moura de Carvalho VicentiniMaria Vitória Lopes Badra Bentley
Jan 31, 2013·Bioconjugate Chemistry·Brittany B LundyPatrick S Stayton
Jun 22, 2011·Nanotoxicology·Gabriella NyitraiJulianna Kardos
Jul 31, 2013·Journal of Pharmaceutical Sciences·Aoife M O'MahonyCaitriona M O'Driscoll
Apr 20, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Antonio NunesKostas Kostarelos
Jan 3, 2012·Biomaterials·Hamidreza M AliabadiHasan Uludağ
May 7, 2015·Chemical Reviews·Jiepin YangYiyun Cheng
Jan 20, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Qi YinYaping Li
Jul 6, 2012·Fundamental & Clinical Pharmacology·Suchi Raghunathan, Bhoomika M Patel
Jul 11, 2014·Molecular Aspects of Medicine·Rui KangDaolin Tang
Jun 19, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hyesun HyunMinhyung Lee
Sep 7, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mary-Louise Rogers, Robert Archer Rush
Oct 21, 2015·Journal of Receptor and Signal Transduction Research·Mohd Imtiaz Nawaz, Ghulam Mohammad
Feb 24, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Olivia M MerkelThomas Kissel
Aug 27, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Chad CurtisElizabeth Nance
Mar 12, 2011·Biomacromolecules·Ana C RodrigoJulián Rodríguez-López
Aug 27, 2016·Biomacromolecules·Alexander C EldredgeZhibin Guan
Jan 1, 2013·Genes·Andrew M Fukuda, Jerome Badaut
Aug 9, 2018·Nanomedicine·Weiyuan ZhangZhiyong He
Jun 15, 2019·Journal of Drug Targeting·Stefanie A PenaAdrien A Eshraghi

❮ Previous
Next ❯

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