Neuroprotection from NMDA excitotoxic lesion by Cu/Zn superoxide dismutase gene delivery to the postnatal rat brain by a modular protein vector.

BMC Neuroscience
Hugo PeluffoBerta Gonzalez

Abstract

Superoxide mediated oxidative stress is a key neuropathologic mechanism in acute central nervous system injuries. We have analyzed the neuroprotective efficacy of the transient overexpression of antioxidant enzyme Cu/Zn Superoxide dismutase (SOD) after excitotoxic injury to the immature rat brain by using a recently constructed modular protein vector for non-viral gene delivery termed NLSCt. For this purpose, animals were injected with the NLSCt vector carrying the Cu/Zn SOD or the control GFP transgenes 2 hours after intracortical N-methyl-D-aspartate (NMDA) administration, and daily functional evaluation was performed. Moreover, 3 days after, lesion volume, neuronal degeneration and nitrotyrosine immunoreactivity were evaluated. Overexpression of Cu/Zn SOD transgene after NMDA administration showed improved functional outcome and a reduced lesion volume at 3 days post lesion. In secondary degenerative areas, increased neuronal survival as well as decreased numbers of degenerating neurons and nitrotyrosine immunoreactivity was seen. Interestingly, injection of the NLSCt vector carrying the control GFP transgene also displayed a significant neuroprotective effect but less pronounced. When the appropriate levels of Cu/Zn SOD are...Continue Reading

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Oct 13, 2007·Pharmaceutical Research·Jamie M BergenSuzie H Pun
Nov 23, 2006·Antioxidants & Redox Signaling·Daoying HuEdda Thiels
Jan 5, 2011·Microbial Cell Factories·Esther Vázquez, Antonio Villaverde
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Jun 20, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Ying ChenJingling Chang
Aug 28, 2007·Annals of Neurology·Hugo PeluffoBerta González
Mar 18, 2010·Journal of Biomolecular Screening·Verónica Toledo-RubioAntonio Villaverde
Mar 31, 2019·Gene Therapy·Daniela Blanco-OcampoHugo Peluffo
May 16, 2019·Neural Regeneration Research·Baruh PolisAbraham O Samson
Mar 25, 2008·Trends in Biotechnology·Neus Ferrer-MirallesAntonio Villaverde
Apr 4, 2009·Biotechnology Advances·Paolo SaccardoNuria González-Montalbán

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Methods Mentioned

BETA
transgenic
transfection
transfect

Software Mentioned

analySIS

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