Neuroprotection in ischemic stroke--combination drug therapy and mild hypothermia in a rat model of permanent focal cerebral ischemia
Abstract
We have recently demonstrated marked neuroprotective efficacy of a combination therapy with magnesium (calcium- and glutamate-antagonist), tirilazad (antioxidant) and mild hypothermia (MTH) in a rat model of transient focal cerebral ischemia. In the present study, we investigated MTH under conditions of permanent focal cerebral ischemia. In part I, 20 Sprague-Dawley rats were subjected to 6 h of permanent, laser-Doppler flowmetry (LDF) controlled middle cerebral artery occlusion (MCAO). Drugs were administered 30 min before and 1 h after MCAO. Hypothermia (33 degrees C) was maintained for 2 h. Infarct size was planimetrically determined after 6 h. In part II, 29 rats were assigned to the same treatment arms and subjected to 7 days of permanent MCAO. Neurological deficits and body weight were assessed daily. Infarct size was determined on day 7. In part I, MTH significantly reduced infarct formation by 52% after 6 h. In part II, high mortality within the first 3 days was observed in both groups. Treated animals showed a significantly better postoperative weight gain on day 7 and neurological recovery on days 6 and 7 compared to controls without significant differences in infarct volume. MTH seems to exert its neuroprotective pro...Continue Reading
References
Citations
Hypothermic protection in an acute hypoxia model in rats: Acid-base and oxidant/antioxidant profiles
Magnesium sulfate neither potentiates nor inhibits tissue plasminogen activator-induced thrombolysis
Nitric oxide induced by ketamine/xylazine anesthesia maintains hepatic blood flow during hypothermia
Postischemic sevoflurane offers no additional neuroprotective benefit to preischemic dexmedetomidine
Temporal effect of acupuncture on amino acid neurotransmitters in rats with acute cerebral ischaemia
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