Neuroprotection of early and short-time applying berberine in the acute phase of cerebral ischemia: up-regulated pAkt, pGSK and pCREB, down-regulated NF-κB expression, ameliorated BBB permeability

Brain Research
Xiaolin ZhangKang Zhao

Abstract

Berberine (BBR) has gained attention for its vast beneficial biological effects through immunomodulation, anti-inflammatory and anti-apoptosis properties. Inflammatory and apoptosis damage play an important role in cerebral ischemic pathogenesis and may represent a target for treatment. The aim of this study was to explore BBR's effect in ischemic injury and the role of the Akt/GSK (glycogen synthase kinase) signaling cascade in mediating the anti-apoptosis and anti-inflammatory effects in the rat brain of permanent middle cerebral artery occlusion (pMCAO). Male Sprague-Dawley rats were subjected to pMCAO and randomly assigned into four groups: Sham (sham-operated) group, pMCAO (pMCAO+0.9% saline) group, BBR-L (pMCAO+BBR 10 mg/kg) and BBR-H (pMCAO+BBR 40 mg/kg) group. BBR was administered immediately after pMCAO and the neuroprotection was detected. Phospho-Akt (pAkt), phospho-glycogen synthase kinase 3-β (pGSK3β), phospho-cAMP response element binding protein (pCREB), nuclear factor-kappa B (NF-κB) and claudin-5 in ischemic cerebral cortex were detected by immunohistochemistry, reverse transcription-polymerase chain reaction and western blotting. Compared with pMCAO group, BBR dramatically lessened neurological deficits scores...Continue Reading

References

May 1, 1979·Journal of Neuropathology and Experimental Neurology·C K Petito
Jan 1, 1989·Stroke; a Journal of Cerebral Circulation·E Z LongaR Cummins
Aug 1, 1998·The Journal of Biological Chemistry·M Pap, G M Cooper
Aug 11, 1999·Trends in Neurosciences·U DirnaglM A Moskowitz
Feb 1, 2000·Trends in Neurosciences·M R Walton, I Dragunow
May 18, 2000·Brain Research. Molecular Brain Research·E A IrvingA A Parsons
Feb 15, 2001·Current Opinion in Neurology·C Iadecola, M Alexander
May 10, 2001·Journal of Molecular and Cellular Cardiology·M ZhangC E Murry
Sep 21, 2001·The Biochemical Journal·S Frame, P Cohen
Oct 5, 2001·Nature Reviews. Molecular Cell Biology·P Cohen, S Frame
Mar 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Karen S Mark, Thomas P Davis
May 3, 2003·Nature Reviews. Neuroscience·Eng H LoMichael A Moskowitz
May 14, 2003·The Journal of Cell Biology·Takehiro NittaShoichiro Tsukita
Jan 21, 2004·Cancer Letters·Chi-Li KuoTsung-Yun Liu
Feb 28, 2004·Stroke; a Journal of Cerebral Circulation·Antti NurmiJari Koistinaho
Mar 24, 2005·Journal of Cellular and Molecular Medicine·Gang SongShideng Bao
Feb 14, 2006·Current Pharmaceutical Design·Morin Christophe, Simon Nicolas
Mar 16, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hidenori EndoPak H Chan
Oct 8, 2008·Neuroscience Letters·Xi-Qiao ZhouXiu-Lan Sun
May 22, 2009·Acta Physiologica Hungarica·F BenaissaMassoud Mahmoudian
Jun 1, 2011·Biochemical and Biophysical Research Communications·Baozhu SunXiumei Zhang
Sep 21, 2011·International Journal of Nanomedicine·Wen TanYitao Wang

❮ Previous
Next ❯

Citations

Nov 17, 2015·Expert Opinion on Therapeutic Targets·Emiliano Peña-AltamiraBarbara Monti
Jul 3, 2015·Experimental and Therapeutic Medicine·Qilin FengYingda Yang
Jun 21, 2015·European Journal of Pharmacology·Anil KumarDinesh K Dhull
Mar 10, 2015·Science China. Life Sciences·WenXiao JiangXiaoJiang Li
Mar 1, 2014·ISRN Neurology·Arshad Majid
May 13, 2017·Journal of Chemical Neuroanatomy·Solmaz Nasseri MalekiFaramarz Souri
Jan 21, 2017·International Journal of Molecular Sciences·Vanessa PorriniMarina Pizzi
Aug 21, 2019·Central Nervous System Agents in Medicinal Chemistry·Anurag K SinghGiriraj T Kulkarni
Feb 11, 2015·Translational Neuroscience·Peng-Fei WangQing-Wu Yang
Sep 5, 2014·Experimental and Therapeutic Medicine·Xiaojuan LiXiaoyin Wang
May 8, 2018·Phytotherapy Research : PTR·Xiaorui Lin, Nan Zhang
Jul 28, 2018·BioMed Research International·Xin-Chen WangShi-Jian Quan
Oct 23, 2016·Journal of Biomedical Science·Cheng-Fu ChangChi-Chen Huang
Apr 6, 2018·International Journal of Molecular Medicine·Si ChenBin Lu
Jun 23, 2018·Chinese Medicine·Jin WangQin-Wan Huang
Aug 11, 2020·European Journal of Pharmacology·Md Sahab UddinLotfi Aleya
May 11, 2021·Frontiers in Pharmacology·Qian XieDaoyin Gong
May 20, 2021·Oxidative Medicine and Cellular Longevity·Hui XuMingfu Wang
Jul 7, 2021·Medical Hypotheses·Saurabh GuptaMohamed M Abdel-Daim
Sep 16, 2021·Journal of Food Biochemistry·Moazzama AkbarMuhammad Ajmal Shah

❮ Previous
Next ❯

Related Concepts

Related Feeds

Acute Stroke

A stroke occurs when blood supply to the brain is interrupted depriving the brain of oxygen and nutrients. This feed focuses cerebrovascular accidents including ischemic and paralytic stroke.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Basal Ganglia Cerebrovascular Disease

Basal ganglia cerebrovascular disease is a condition where the blood vessels in the basal ganglia are damaged or malfunction. Discover the latest research on basal ganglia cerebrovascular disease here.

Blood Brain Barrier & Cytokines

Some cytokines are able to cross the blood brain barrier through transport systems and enter the cerebrospinal fluid and interstitial fluid spaces. Here is the latest research on cytokines crossing the blood brain barrier and how this can affect tissues within the CNS.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.