Neuroprotection of edaravone on hypoxic-ischemic brain injury in neonatal rats

Brain Research. Developmental Brain Research
Noriko YasuokaGoro Takada

Abstract

Edaravone has an inhibitory effect on lipid peroxidation by scavenging free radicals and prevents vascular endothelial cell injury. We examined whether edaravone was effective on hypoxic-ischemic (HI) brain injury in immature brain or not using the Rice-Vannucci model. The initial dose, 3 mg/kg (0.05 ml) of edaravone, was injected intraperitoneally just before hypoxic exposure. Subsequently, the same dose was injected every 12 h until the animals were killed. Controls received saline injection as the same protocol. Macroscopic evaluation of brain injury revealed that the neuroprotective effect of edaravone on HI brain after 48 h post HI. TUNEL showed that edaravone injection decreased neurodegeneration. Quantitative analysis of cell death using H&E-stained 2.5 microm sections showed that there was a trend for both necrotic and apoptotic cells to decrease in edaravone injection group. Edaravone injection inhibited the release of cytochrome c from mitochondria to cytosol and caspase-3 activation in cortex and hippocampus between 24 and 168 h post HI. Our results suggest that edaravone is protective after HI insult in the immature brain by decreasing both apoptosis and necrosis and also by inhibiting mitochondrial injury.

References

Apr 1, 1992·Toxicology Letters·R BoseV Zitko
Jan 1, 1990·Annals of the New York Academy of Sciences·S MurotaN Suda
Apr 1, 1988·Stroke; a Journal of Cerebral Circulation·K AbeK Kogure
Jul 1, 1988·Prostaglandins, Leukotrienes, and Essential Fatty Acids·T WatanabeS Murota
Feb 1, 1981·Annals of Neurology·J E RiceJ B Brierley
Mar 1, 1994·Archives of Disease in Childhood. Fetal and Neonatal Edition·T E InderB J Taylor
Feb 1, 1996·Acta Physiologica Scandinavica·S KurodaB K Siesjö
Feb 3, 1997·The Journal of Comparative Neurology·C Portera-CailliauL J Martin
Jul 1, 1997·Brain & Development·W H TrescherM V Johnston
Sep 26, 1997·The Journal of Comparative Neurology·R W Guillery, K Herrup
Dec 31, 1997·Pediatrics·R C Vannucci, J M Perlman
Mar 14, 1998·Experimental Cell Research·C A Messam, R N Pittman
May 20, 1998·Journal of the Neurological Sciences·Y LiM Chopp
Nov 11, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M FujimuraP H Chan
Apr 22, 1999·Annals of Neurology·J B SchulzM A Moskowitz
Nov 4, 2000·Journal of Neurotrauma·J P MacManus, A M Buchan
May 30, 2001·FEBS Letters·A AtlanteS Passarella
Jun 1, 2001·Pediatric Research·M V JohnstonW Nakajima
Oct 13, 2001·Developmental Neuroscience·F J NorthingtonL J Martin
May 31, 2002·Pharmacological Reviews·Yossi Gilgun-SherkiDaniel Offen
May 6, 2003·Biochemical and Biophysical Research Communications·Klas BlomgrenHenrik Hagberg

❮ Previous
Next ❯

Citations

Mar 23, 2005·Acta Pharmacologica Sinica·Li-hui Zhang, Er-qing Wei
Apr 25, 2009·Pediatric Research·Richard L Auten, Jonathan M Davis
Mar 3, 2007·Biological & Pharmaceutical Bulletin·Kenichi WatanabeYoshifusa Aizawa
Aug 16, 2006·Journal of Zhejiang University. Science. B·Ying SongEr-qing Wei
Jul 29, 2009·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Masao WatanabeTatsuo Yamamoto
Nov 23, 2006·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Naoki MasaokaNorimichi Nemoto
Jul 6, 2010·European Journal of Pharmacology·Zhenhua WangQiusheng Zheng
Aug 7, 2008·Brain & Development·Shinobu MiuraTsutomu Takahashi
Sep 12, 2006·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Yumi Nakano-OkudaToru Sugimoto
Oct 12, 2005·Brain Research. Brain Research Reviews·Frances J NorthingtonLee J Martin
Jul 25, 2015·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Hidetoshi NakamotoYoshikazu Okada
Aug 3, 2006·The American Journal of Chinese Medicine·Haruyo IchikawaTetsuya Konishi
Apr 25, 2015·Behavioral and Brain Functions : BBF·Guojuan LiangJunqing Yang
Jan 20, 2021·Physiological Research·A FrajewickiV Riljak

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.