Neuroprotective effect of grape seed extract on brain ischemia: a proteomic approach

Metabolic Brain Disease
Safwen KadriMeherzia Mokni

Abstract

Stroke is one of the leading causes of long-lasting disability in human and oxidative stress an important underlying cause. Molecular insights into pathophysiology of ischemic stroke are still obscure, and the present study investigated the protective effect of high dosage Grape Seed Extract (GSE 2.5 g/kg) on brain ischemia-reperfusion (I/R) injury using a proteomic approach. Ischemia was realized by occlusion of the common carotid arteries for 30 min followed by 1 h reperfusion on control or GSE pre-treated rats, and a label-free quantification followed by mass spectrometry analysis used to evaluate I/R induced alterations in protein abundance and metabolic pathways as well as the protection afforded by GSE. I/R-induced whole brain ionogram dyshomeostasis, ultrastructural alterations, as well as inflammation into hippocampal dentate gyrus area, which were evaluated using ICP-OES, transmission electron microscopy and immuno-histochemistry respectively. I/R altered the whole brain proteome abundance among which 108 proteins were significantly modified (35 up and 73 down-regulated proteins). Eighty-four proteins were protected upon GSE treatment among which 27 were up and 57 down-regulated proteins, suggesting a potent protective...Continue Reading

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Citations

Jul 7, 2019·Translational Stroke Research·Haiying LiGang Chen
Sep 7, 2020·Journal of Cosmetic Dermatology·Liudmila YarovayaWatcharee Khunkitti

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Methods Mentioned

BETA
nuclear translocation
CLIP

Software Mentioned

Progenesis LC - MS
Matrix Science
R Studio
David
MASCOT
Cytoscape
Gene MANIA Cytoscape

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