Neuroprotective effect of menaquinone-4 (MK-4) on transient global cerebral ischemia/reperfusion injury in rat

PloS One
Bahram Farhadi Moghadam, Masoud Fereidoni

Abstract

Cerebral ischemia/reperfusion (I/R) injury causes cognitive deficits, excitotoxicity, neuroinflammation, oxidative stress and brain edema. Vitamin K2 (Menaquinone 4, MK-4) as a potent antioxidant can be a good candidate to ameliorate I/R consequences. This study focused on the neuroprotective effects of MK-4 for cerebral I/R insult in rat's hippocampus. The rat model of cerebral I/R was generated by transient bilateral common carotid artery occlusion for 20 min. Rats were divided into control, I/R, I/R+DMSO (solvent (1% v/v)) and I/R+MK-4 treated (400 mg/kg, i.p.) groups. Twenty-four hours after I/R injury induction, total brain water content, superoxide dismutase (SOD) activity, nitrate/nitrite concentration and neuronal density were evaluated. In addition to quantify the apoptosis processes, TUNEL staining, as well as expression level of Bax and Bcl2, were assessed. To evaluate astrogliosis and induced neurotoxicity by I/R GFAP and GLT-1 mRNA expression level were quantified. Furthermore, pro-inflammatory cytokines including IL-1β, IL-6 and TNF-α were measured. Seven days post I/R, behavioral analysis to quantify cognitive function, as well as Nissl staining for surviving neuronal evaluation, were conducted. The findings indi...Continue Reading

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Citations

May 21, 2020·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·Pengyu GongYingdong Zhang
Dec 12, 2020·Psychoneuroendocrinology·Thomaz F S BastiaanssenJohn F Cryan
Feb 23, 2021·Frontiers in Aging Neuroscience·Yi YaoJianzhao Luo
Aug 28, 2021·Nutrients·Halima SultanaHitoshi Shirakawa

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Enzyme-linked immunosorbent assay

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