Abstract
The purpose of this study is to investigate the protective effect and molecular mechanism of the sulfated polysaccharide (SJP) isolated from the sea cucumber Stichopus japonicus against 6-OHDA-induced toxicity in SH-SY5Y cells. The results showed that SJP could protect SH-SY5Y cells against 6-OHDA-induced cell injury. We found that SJP effectively improves cell viability, decreases LDH leakage, and reverses morphological damage. Moreover, SJP significantly increases SOD activity but decreases MDA levels and ROS generation. Effect of SJP on 6-OHDA-induced cell death in SH-SY5Y cells is associated with an arrest in the G1/S phase of the cell cycle and inhibits the expression of Cyclin D3. 6-OHDA-induced intracellular generation of ROS and mitochondrial dysfunctions, release of cytochrome c, imbalance of Bax/Bcl-2, cleaved caspase-9/caspase-9 and cleaved caspase-3/caspase-3 ratio, and p-p53 activation were strikingly attenuated by SJP pretreatment. Meanwhile, SJP counteracted NF-κB activation, thereby preventing up-regulation of iNOS and intracellular NO release. The data provide the first evidence that SJP protects SH-SY5Y cells against 6-OHDA toxicity possibly by inhibiting MAPK and NF-κB and activating PI3K/Akt signaling pathwa...Continue Reading
References
Feb 13, 2001·Trends in Neurosciences·A CopaniM Memo
Mar 4, 2003·Journal of Cellular and Molecular Medicine·T Wakabayashi
Mar 7, 2006·Journal of Neurochemistry·Mingxing Ouyang, Xun Shen
Apr 10, 2009·Current Medicinal Chemistry·Junichi MaruyamaHidenori Ichijo
Apr 2, 2013·Archives of Toxicology·Krishnendu SinhaParames C Sil
Feb 7, 2014·BMB Reports·Seung-Wook Chi
Sep 1, 2014·Cellular Signalling·David Heras-SandovalJosé Pedraza-Chaverri
Oct 2, 2014·Chemical Research in Toxicology·Chia-Yuan LinChia-Wen Tsai
Sep 1, 2015·Molecular BioSystems·Jing GaoBin Yi
Citations
Aug 27, 2016·Carbohydrate Polymers·Opeyemi J OlatunjiZhaoliang Su
Jun 22, 2017·Food & Function·Tiange LiXueying Mao
Feb 28, 2018·Environmental Toxicology·Su Eun ChoiHyun Chul Koh
Sep 22, 2016·Neural Regeneration Research·Qun CaiXing Fen
Sep 19, 2019·Journal of Applied Microbiology·L ChenG-Y Wang
Apr 9, 2020·Pharmaceutics·Nadia FerlazzoMichele Navarra
Oct 16, 2018·RSC Advances·A MohankumarP Sundararaj
Jul 30, 2017·Nutrients·Haifeng LiZebo Huang
Dec 5, 2019·Marine Drugs·Qiwu ZhongHong Wang
Nov 26, 2016·Oxidative Medicine and Cellular Longevity·Haifeng LiZebo Huang
Aug 9, 2017·Oxidative Medicine and Cellular Longevity·Xiao-Tao MaJian-Ming Ouyang
Feb 6, 2019·Marine Drugs·Joana SilvaRui Pedrosa
Jun 7, 2018·Frontiers in Molecular Neuroscience·Huichun TongShaogang Qu
Apr 3, 2019·Marine Drugs·Ruowei GuanJinhua Zhao
Jan 14, 2021·International Journal of Biological Macromolecules·Marjan TalebiSaeed Samarghandian
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Nawaphat MalaiwongKrai Meemon
Mar 17, 2021·Critical Reviews in Food Science and Nutrition·Devina LobineMohamad Fawzi Mahomoodally
May 17, 2021·Food Research International·Xiaomei WangNianjun Xu