Neuroprotective effect of sulfated polysaccharide isolated from sea cucumber Stichopus japonicus on 6-OHDA-induced death in SH-SY5Y through inhibition of MAPK and NF-κB and activation of PI3K/Akt signaling pathways

Biochemical and Biophysical Research Communications
Chao CuiAiguo Ji

Abstract

The purpose of this study is to investigate the protective effect and molecular mechanism of the sulfated polysaccharide (SJP) isolated from the sea cucumber Stichopus japonicus against 6-OHDA-induced toxicity in SH-SY5Y cells. The results showed that SJP could protect SH-SY5Y cells against 6-OHDA-induced cell injury. We found that SJP effectively improves cell viability, decreases LDH leakage, and reverses morphological damage. Moreover, SJP significantly increases SOD activity but decreases MDA levels and ROS generation. Effect of SJP on 6-OHDA-induced cell death in SH-SY5Y cells is associated with an arrest in the G1/S phase of the cell cycle and inhibits the expression of Cyclin D3. 6-OHDA-induced intracellular generation of ROS and mitochondrial dysfunctions, release of cytochrome c, imbalance of Bax/Bcl-2, cleaved caspase-9/caspase-9 and cleaved caspase-3/caspase-3 ratio, and p-p53 activation were strikingly attenuated by SJP pretreatment. Meanwhile, SJP counteracted NF-κB activation, thereby preventing up-regulation of iNOS and intracellular NO release. The data provide the first evidence that SJP protects SH-SY5Y cells against 6-OHDA toxicity possibly by inhibiting MAPK and NF-κB and activating PI3K/Akt signaling pathwa...Continue Reading

References

Mar 4, 2003·Journal of Cellular and Molecular Medicine·T Wakabayashi
Apr 10, 2009·Current Medicinal Chemistry·Junichi MaruyamaHidenori Ichijo
Apr 2, 2013·Archives of Toxicology·Krishnendu SinhaParames C Sil

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Citations

Sep 19, 2019·Journal of Applied Microbiology·L ChenG-Y Wang
Nov 26, 2016·Oxidative Medicine and Cellular Longevity·Haifeng LiZebo Huang
Jan 14, 2021·International Journal of Biological Macromolecules·Marjan TalebiSaeed Samarghandian
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Nawaphat MalaiwongKrai Meemon
Mar 17, 2021·Critical Reviews in Food Science and Nutrition·Devina LobineMohamad Fawzi Mahomoodally
May 17, 2021·Food Research International·Xiaomei WangNianjun Xu

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