Neuroprotective effects of 17beta-estradiol and nonfeminizing estrogens against H2O2 toxicity in human neuroblastoma SK-N-SH cells

Molecular Pharmacology
Xiaofei WangJames W Simpkins

Abstract

Neuroprotective effects of estrogens have been shown in various in vitro and in vivo models, but the mechanisms underlying protection by estrogen are not clear. Mounting evidence suggests antioxidant effects contribute to the neuroprotective effects of estrogens. In the present study, we assessed the protective effects of estrogens against H2O2-induced toxicity in human neuroblastoma cells and the potential mechanisms involved in this protection. We demonstrate that 17beta-estradiol (17beta-E2) increases cell survival against H2O2 toxicity in human neuroblastoma cells. 17beta-E2 effectively reduced lipid peroxidation induced by 5-min H2O2 exposure. Furthermore, 17beta-E2 exerts the protective effects by maintaining intracellular Ca2+ homeostasis, attenuating ATP depletion, ablating mitochondrial calcium overloading, and preserving mitochondrial membrane potential. Two nonfeminizing estrogens, 17alpha- and ent-estradiol, were as effective as 17beta-E2 in increasing cell survival, alleviating lipid peroxidation, preserving mitochondrial function, and maintaining intracellular glutathione levels and Ca2+ homeostasis against H2O2 insult. Moreover, the estrogen receptor antagonist fulvestrant (ICI 182,780) did not block effects of 1...Continue Reading

References

May 1, 1990·The American Journal of Physiology·T E Gunter, D R Pfeiffer
Jan 1, 1983·Annual Review of Biochemistry·A Meister, M E Anderson
Nov 13, 1995·Biochemical and Biophysical Research Communications·C BehlF Holsboer
Aug 1, 1994·Molecular and Cellular Neurosciences·J Bishop, J W Simpkins
Feb 1, 1996·Obstetrics and Gynecology·B B Sherwin
Jul 1, 1996·Journal of the American Geriatrics Society·S J Birge
Jul 5, 1996·Neuroscience Letters·C A SingerD M Dorsa
Mar 24, 1997·FEBS Letters·T LópezA Ortega
Jan 28, 1998·Stroke; a Journal of Cerebral Circulation·N J AlkayedP D Hurn
Feb 12, 1998·The Journal of Steroid Biochemistry and Molecular Biology·P S GreenJ W Simpkins
Mar 13, 1998·JAMA : the Journal of the American Medical Association·K YaffeD Grady
Oct 17, 1998·Toxicology and Applied Pharmacology·D Blum-DegenM E Götz
Nov 11, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D B DubalP M Wise
Dec 8, 1998·Journal of Neuroscience Research·H SawadaS Shimohama
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·B Moosmann, C Behl
Nov 24, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·C CulmseeJ Krieglstein
May 19, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·P S Green, J W Simpkins
May 23, 2000·Journal of Neurochemistry·Y J KimK T Kim
Jul 15, 2000·Journal of Korean Medical Science·Y H BaeJ Y Koh
Feb 7, 2001·Journal of Neurochemistry·L A De GirolamoE E Billett
Jun 27, 2001·Journal of Neurocytology·P S GreenJ W Simpkins
Jul 17, 2001·Current Opinion in Cell Biology·S P YuD W Choi
Dec 18, 2001·Brain Research. Brain Research Reviews·V D RamirezI Joe

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Citations

Apr 13, 2010·Cancer Chemotherapy and Pharmacology·Valentina K TodorovaV Suzanne Klimberg
Apr 23, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Eun-Sook Y LeeMichael Aschner
Dec 4, 2008·The Journal of Pharmacology and Experimental Therapeutics·Marianna E JungJames W Simpkins
Jun 16, 2011·International Journal of Molecular Sciences·Jakob O StromElvar Theodorsson
Sep 24, 2014·Brain Research Bulletin·Mrinmay ChakrabartiSwapan K Ray
Dec 14, 2011·Neural Plasticity·Ignacio González-BurgosLuis Miguel Garcia-Segura
Oct 22, 2015·Canadian Journal of Physiology and Pharmacology·Yuan ChengJianjun Zhao
Nov 6, 2014·PloS One·Gwendolyn I HumphreysAnn M Nardulli
Sep 16, 2016·Oxidative Medicine and Cellular Longevity·Israel Pérez-TorresNatalia Pavón
Sep 6, 2008·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Donald R Branch
Jan 4, 2008·American Journal of Physiology. Endocrinology and Metabolism·James M FlynnPatrick R Cammarata
Jul 22, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tokutaro YamaguchiTae-Jin Oh
May 21, 2009·Journal of Neurochemistry·Eun-Sook Y LeeMichael Aschner
Jul 27, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marianna E Jung, Daniel B Metzger
Jun 15, 2012·Journal of Biochemical and Molecular Toxicology·Dilek Taskiran, Vedat Evren
Jul 21, 2007·Endocrine Reviews·Rainer H Straub
Oct 29, 2013·Journal of Pharmacological Sciences·Ryosuke TanakaYasuo Matsumura
Nov 21, 2017·Translational Stroke Research·Mohammad KhaksariNader Shahrokhi
Jul 6, 2019·Scientific Reports·Linaloe Guadalupe Manzano PechIsrael Pérez-Torres
Nov 2, 2013·Scientific Reports·Jakob O StromAnnette Theodorsson
Mar 9, 2017·Journal of Alzheimer's Disease : JAD·Ce ZhangYuanlin Dong
Jul 11, 2020·International Journal of Molecular Sciences·Yvette Akwa
Dec 23, 2020·Nutrients·Saioa Gómez-ZoritaMaría Puy Portillo
Dec 31, 2020·International Journal of Molecular Sciences·Erzsébet KövesdiIstván M Abrahám
Mar 20, 2021·Journal of Inflammation Research·Xiaoyu HangFeihu Chen
May 22, 2007·Brain Research Reviews·James W Simpkins, James A Dykens
May 19, 2009·Psychoneuroendocrinology·Lydia L DonCarlosLuis M Garcia-Segura
Nov 26, 2009·Biochimica Et Biophysica Acta·James W SimpkinsJames A Dykens

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