Neuroprotective effects of ebselen are associated with the regulation of Bcl-2 and Bax proteins in cultured mouse cortical neurons

Neuroscience Letters
Jie-Hua XuZhang-Jin Zhang

Abstract

There is little information available on the mechanisms underlying the neuroprotective actions of the organoselenium compound ebselen. In this study, we sought to determine the relationship between alterations in the expression of Bcl-2 and Bax proteins and intracellular levels of calcium and the protective effects of ebselen with a concentration range of 0.01-20 microM against glutamate toxicity in cultured mouse cortical neurons. Pretreatment with ebselen at moderate doses (4-12 microM), but not at lower or higher doses, significantly improved glutamate-induced suppression of cell viability. Pretreatment with ebselen (8 microM) also prevented apoptotic alterations, completely reversed the suppression of Bcl-2 expression, and significantly inhibited Bax overexpression, but did not alter elevated intracellular concentrations of calcium induced by glutamate. Pre-, co-, and post-treatment with ebselen (8 microM) had similar potency in improving the decreased viability of glutamate-exposed cells. These results indicate that the neuroprotective effects of ebselen at low doses are associated with the regulation of Bcl-2 and Bax proteins but appear to be independent of glutamate-mediated elevation of intracellular calcium, suggesting...Continue Reading

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Citations

Jul 2, 2011·Archives of Toxicology·Cristina W Nogueira, João B T Rocha
Sep 11, 2012·The Journal of Nutritional Biochemistry·Simone PintonCristina Wayne Nogueira
May 18, 2007·Mutation Research·Renato Moreira RosaJoão Antonio Pêgas Henriques
Apr 19, 2016·Chemico-biological Interactions·Michael CostaMaria E Pereira
Feb 11, 2015·Neurobiology of Aging·Daniela PuzzoAgostino Palmeri
May 29, 2014·Molecular Biology Reports·Gajendra Kumar Azad, Raghuvir S Tomar

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