Neuroprotective effects of erythropoietin posttreatment against kainate-induced excitotoxicity in mixed spinal cultures

Journal of Neuroscience Research
Jong Yoon YooHea Nam Hong

Abstract

Although the neuroprotective effects of erythropoietin (EPO) preconditioning are well known, the potential of postapplied EPO to protect neurons against excitotoxic injury has not been clearly established. Here we show that kainate (KA)-induced excitotoxicity, which plays a key role in secondary spinal cord injury, decreased neuron survival, inhibited neurite extension, and significantly reduced the expression of erythropoietin receptors (EpoR) in cultured spinal neurons. Posttreatment with EPO for 48 hr protected neurons against KA-induced injury, opposing KA-induced apoptosis and promoting regrowth of motoneuron neurites. These neuroprotective effects were paralleled by a restoration of EpoR expression. The importance of the EpoR signaling pathway was demonstrated using an EpoR blocking antibody, which neutralized the neuroprotective action of EPO posttreatment and prevented EPO-induced increases in EpoR expression. We also found that up-regulated EpoR stimulated the Janus kinase 2 (JAK2) pathway, which is known to facilitate neuronal growth and neurite regeneration. Although EPO posttreatment modestly attenuated KA-induced reactive gliosis in mixed neuron-glial cultures, blocking EpoR activity did not alter glial fibrillary ...Continue Reading

References

Jun 1, 1988·Annals of Neurology·A I Faden, R P Simon
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·M DigicayliogluM Gassmann
Jan 7, 1998·Trends in Neurosciences·J L RidetF H Gage
Jun 12, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M BernaudinE Petit
Sep 14, 2000·Proceedings of the National Academy of Sciences of the United States of America·M L BrinesA Cerami
Sep 23, 2000·Brain Research. Molecular Brain Research·K ChinC T Noguchi
Nov 25, 2000·Journal of the Neurological Sciences·L Van Den BoschW Robberecht
Apr 20, 2001·Acta Neuropathologica·A L SirénH Ehrenreich
Oct 5, 2001·Nature Reviews. Molecular Cell Biology·M P Scheid, J R Woodgett
Apr 2, 2002·Experimental Neurology·L Van Den BoschM W Berchtold
Oct 3, 2002·Journal of Neurochemistry·Carol L Poitry-YamateThomas Rauen
Mar 6, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jill R FaulknerMichael V Sofroniew
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Murat DigicayliogluStuart A Lipton
Jan 6, 2005·JAMA : the Journal of the American Medical Association·Kenneth MaieseZhao Zhong Chong
Jun 7, 2005·Molecular and Cellular Neurosciences·Alexandra KretzStefan Isenmann
Jul 7, 2005·Journal of Neurochemistry·Céline VermeirenEmmanuel Hermans
Oct 27, 2005·Blood·Steve ElliottC Glenn Begley
Jan 27, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Peter T TsaiS Thomas Carmichael
May 26, 2007·Journal of Neuroscience Methods·Agnete KirkebyLone Helboe
Jul 7, 2007·The European Journal of Neuroscience·V R KingA T Michael-Titus

❮ Previous
Next ❯

Citations

Aug 25, 2010·Psychopharmacology·Pascal Jean Denis GoetghebeurRebecca Dias
May 15, 2010·Molecular Neurobiology·Yan DongAlbert Cheung Hoi Yu
Sep 29, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kenneth MaieseYan Chen Shang
Aug 19, 2010·Oxidative Medicine and Cellular Longevity·Kenneth MaieseYan Chen Shang
Aug 25, 2010·Pharmacology & Therapeutics·L VellyP Pisano
Mar 12, 2010·Journal of Clinical Pharmacology·Kenneth MaieseJinling Hou
Mar 21, 2013·Expert Opinion on Therapeutic Targets·Zhao Zhong ChongKenneth Maiese
Dec 27, 2011·Biochemical and Biophysical Research Communications·Yun Kyung ChoHea Nam Hong
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zohreh HosseinzadehFlorian Lang
Jun 17, 2011·Anaesthesia and Intensive Care·E M MooreA D Nichol
Oct 26, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Wei ChenZahid Hussain
Oct 6, 2010·World Neurosurgery·Jay D TurnerCharles J Prestigiacomo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Astrocytes & Neurodegeneration

Astrocytes are important for the health and function of the central nervous system. When these cells stop functioning properly, either through gain of function or loss of homeostatic controls, neurodegenerative diseases can occur. Here is the latest research on astrocytes and neurodegeneration.

Astrocytes in Repair & Regeneration

Astrocytes are glial cells found within the CNS and are able to regenerate new neurons. They become activated during CNS injury and disease. The activation leads to the transcription of new genes and the repair and regeneration of neurons. Discover the latest research on astrocytes in repair and regeneration here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis