Neuroprotective effects of oligodendrocyte progenitor cell transplantation in premature rat brain following hypoxic-ischemic injury

PloS One
Long-Xia ChenJin Li

Abstract

Periventricular leukomalacia (PVL) is a common ischemic brain injury in premature infants for which there is no effective treatment. The objective of this study was to determine whether transplanted mouse oligodendrocyte progenitor cells (OPCs) have neuroprotective effects in a rat model of PVL. Hypoxia-ischemia (HI) was induced in 3-day-old rat pups by left carotid artery ligation, followed by exposure to 6% oxygen for 2.5 h. Animals were assigned to OPC transplantation or sham control groups and injected with OPCs or PBS, respectively, and sacrificed up to 6 weeks later for immunohistochemical analysis to investigate the survival and differentiation of transplanted OPCs. Apoptosis was evaluated by double immunolabeling of brain sections for caspase-3 and neuronal nuclei (NeuN), while proliferation was assessed using a combination of anti-Nestin and -bromodeoxyuridine antibodies. The expression of brain-derived neurotrophic factor (BDNF) and Bcl-2 was examined 7 days after OPC transplantation. The Morris water maze was used to test spatial learning and memory. The results showed that transplanted OPCs survived and formed a myelin sheath, and stimulated BDNF and Bcl-2 expression and the proliferation of neural stem cells (NSC),...Continue Reading

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Citations

Sep 22, 2015·International Journal of Molecular Sciences·Brandon J DixonJohn H Zhang
Nov 28, 2015·Archivum Immunologiae Et Therapiae Experimentalis·Nan Xiao, Quynh-Thu Le
Apr 20, 2016·Progress in Neurobiology·Yuan WangGuodong Cao
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Mar 24, 2018·Pflügers Archiv : European journal of physiology·Kana OhashiShuji Kaneko
Nov 12, 2020·Expert Review of Neurotherapeutics·Francesco PisaniCarlotta Spagnoli

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