Neuroprotective effects of orientin on hydrogen peroxide‑induced apoptosis in SH‑SY5Y cells

Molecular Medicine Reports
Benjamin Ngee Tiing LawYing Pei Wong

Abstract

Neurodegenerative diseases remain a global issue which affects the ageing population. Efforts towards determining their aetiologies to understand their pathogenic mechanisms are underway in order to identify a pathway through which therapeutic measures can be applied. One such pathogenic mechanism, oxidative stress (OS), is widely considered to be involved in neurodegenerative disease. Antioxidants, most notably flavonoids, have promising potential for therapeutic use as shown in in vitro and in vivo studies. In view of the importance of flavonoids for combating OS, this study investigated the neuroprotective effects of orientin, which has been reported to be capable of crossing the blood‑brain barrier. The maximum non‑toxic dose (MNTD) of orientin against SH‑SY5Y neuroblastoma cells was determined using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay. The effects of the MNTD and the half MNTD (½MNTD) of orientin on cell cycle progression and intracellular reactive oxygen species (ROS) levels, as well as the activity of caspases 3/7, 8 and 9 after exposure to 150 µM of hydrogen peroxide (H2O2) were also determined using flow cytometry, a 2',7'‑dichlorodihydrofluorescein‑diacetate (DCFH‑DA) assay and c...Continue Reading

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Citations

Jun 15, 2016·Advances in Pharmacological Sciences·Kit Ying LamYee How Say
Feb 2, 2016·Free Radical Biology & Medicine·Lorenzo Di Cesare MannelliPaola Failli
Oct 27, 2016·Oxidative Medicine and Cellular Longevity·Alencar Kolinski MachadoIvana Beatrice Mânica da Cruz
May 26, 2018·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Fatma GuesmiAhmed Landoulsi
Feb 21, 2019·Journal of Medicinal Food·Kenji KuriyaHayato Umekawa
Aug 20, 2015·Oncotarget·Davide Guerrieri, Henriette van Praag
May 21, 2021·Saudi Journal of Biological Sciences·Abdelgawad A FahmiMansour Sobeh

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