Neuroprotective effects of orientin on oxygen-glucose deprivation/reperfusion-induced cell injury in primary culture of rat cortical neurons

Experimental Biology and Medicine
Tian TianLi Liu

Abstract

Orientin (luteolin-8-C-glucoside) is a phenolic compound found abundantly in millet, juice, and peel of passion fruit and has been shown to have antioxidant properties. In the present study, we explored the effects of orientin on oxygen-glucose deprivation/reperfusion (OGD/RP)-induced cell injury in primary culture of rat cortical neurons using an in vitro model of neonatal ischemic brain injury. The reduced cell viability and elevated lactate dehydrogenase leakage were observed after OGD/RP exposure, which were then reversed by orientin (10, 20, and 30 µM) pretreatment in a dose-dependent manner. Additionally, OGD/RP treatment resulted in significant oxidative stress, accompanied by enhanced intracellular reactive oxygen species (ROS) generation, and obvious depletion in the activities of intracellular Mn-superoxide dismutase, catalase, and glutathione peroxidase antioxidases. However, these effects were dose dependently restored by orientin pretreatment. We also found that orientin pretreatment dose dependently suppressed [Ca2+]i increase and mitochondrial membrane potential dissipation caused by OGD/RP in primary culture of rat cortical neurons. Western blot analysis showed that OGD/RP exposure induced a distinct decrease of...Continue Reading

References

Mar 5, 1999·Mutation Research·L J Marnett
Jul 6, 2000·Progress in Neurobiology·R Dringen
Oct 7, 2000·Radiation Research·P Uma DeviM K Unnikrishnan
Jan 10, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·P H Chan
Jun 1, 2001·Pediatric Research·M V JohnstonW Nakajima
Sep 21, 2001·Biochemical and Biophysical Research Communications·A IkariY Suketa
May 7, 2002·Biochemical Pharmacology·Domenico Praticò
Apr 9, 2008·Journal of Pharmaceutical and Biomedical Analysis·Deqiang LiKunfeng Duan
May 5, 2009·The Journal of Biological Chemistry·Melissa Brazier-HicksRobert Edwards
Apr 27, 2011·Annals of Neurology·Frances J NorthingtonLee J Martin
Feb 9, 2012·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Serafina PerroneGiuseppe Buonocore
Jan 30, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mario J SimirgiotisEdward J Kennelly
Dec 25, 2013·Molecular Medicine Reports·Benjamin Ngee Tiing LawYing Pei Wong
Jan 29, 2014·TheScientificWorldJournal·Shashank Kumar, Abhay K Pandey
Feb 12, 2014·Antioxidants & Redox Signaling·Rodrigo Portes UreshinoSoraya Soubhi Smaili
Aug 5, 2014·Biological & Pharmaceutical Bulletin·Xiang ZhouXuechu Zhen
Dec 3, 2014·International Journal of Biological Macromolecules·Yunliang XieYingjie Zhang
Jan 30, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Beatrice D'OrsiJochen H M Prehn
Mar 20, 2015·Molecular Nutrition & Food Research·Yi LiuYinghua Yu
Sep 24, 2015·Behavioural Brain Research·Shuting WangYi Liu
Feb 16, 2016·European Journal of Pharmacology·Liya LiuXiulan Huang
Apr 14, 2016·Oxidative Medicine and Cellular Longevity·Phuong Anh Nguyen ThiLu Xie
Jul 21, 2016·Journal of Ethnopharmacology·Erminiana Daminani MendonçaAlexandre de Barros Falcão Ferraz

❮ Previous
Next ❯

Citations

Dec 7, 2018·Current Neuropharmacology·Nicolás Toro-UrregoFrancisco Capani
Jun 28, 2019·Journal of Molecular Neuroscience : MN·Yi ZhongNing Jia
May 15, 2019·Cells·Edoardo ParrellaMarina Pizzi
Nov 11, 2020·Nutritional Neuroscience·Diulie Valente de SouzaAlencar Kolinski Machado

❮ Previous
Next ❯

Methods Mentioned

BETA
pharmacotherapy
flow cytometry

Software Mentioned

Gel
Pro Analyzer
SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.