Neuroprotective effects of pretreatment with propofol in LPS-induced BV-2 microglia cells: role of TLR4 and GSK-3β.

Inflammation
Bo GuiYanning Qian

Abstract

Surgery often leads to neuroinflammation, which mainly acts as the activation of microglia cells. Propofol is always used for induction and maintenance of anesthesia prior to surgical trauma, whereas whether or not it could attenuate neuroinflammation used prophylactically is not well defined. In the present study, we incubated BV-2 microglia cells with 1 μg/ml lipopolysaccharide (LPS) to mimic neuroinflammation in vitro. Firstly, cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and the data indicated that propofol would not reduce cell viability unless its concentration reached 300 μM. Secondly, BV-2 microglia cells were pretreated with 30 μM propofol (clinically relevant concentration), and then stimulated with LPS. The results showed that the production of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-10 was considerably increased by LPS, but the change could be markedly attenuated by pretreatment with propofol. Meanwhile, pretreatment with propofol inhibited LPS-induced augmentation of toll-like receptor 4 (TLR4) expression at both mRNA and protein levels and further upregulated LPS-induced inactivation of glycogen synthase kinase-3β (GSK-3β) in BV-2 m...Continue Reading

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Citations

Mar 21, 2013·Journal of Physiology and Biochemistry·Xia QinYong-Mei Zhang
Feb 11, 2014·International Immunopharmacology·Yubing GuoZhongdong Qiao
Mar 13, 2014·Trends in Microbiology·Huizhi WangDavid A Scott
Oct 2, 2014·Neurochemical Research·Shenghua ZhuXin-Min Li
Apr 24, 2015·International Journal of Molecular Sciences·Chingju LinDah-Yuu Lu
Sep 21, 2018·Experimental and Therapeutic Medicine·Jian WangJianchun Li
Dec 23, 2014·Medical Science Monitor Basic Research·Hongquan DongYanning Qian
Jul 4, 2018·Journal of Alzheimer's Disease : JAD·Bingyu LiDanhong Chen
Feb 16, 2017·Neural Regeneration Research·Hu LiuXue-Sheng Liu
Mar 23, 2019·Journal of Neuroinflammation·Lindsay M ParkerNicolle H Packer
Sep 26, 2020·Frontiers in Cellular Neuroscience·Wenguo FanHongwen He
May 22, 2021·Frontiers in Immunology·Ricarda Cortés-VieyraVíctor M Baizabal-Aguirre
Jul 3, 2021·International Journal of Molecular Sciences·Rezan Ashayeri AhmadabadMaryam Khaleghi Ghadiri
Aug 24, 2021·Journal of Immunology Research·Xi XiaoSihua Qi

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