Neuroprotective effects of the Phellinus linteus ethyl acetate extract against H2O2-induced apoptotic cell death of SK-N-MC cells

Nutrition Research
Doo Jin ChoiYong Il Park

Abstract

Numerous studies have suggested that neuronal cells are protected against oxidative stress-induced cell damage by antioxidants, such as polyphenolic compounds. Phellinus linteus (PL) has traditionally been used to treat various symptoms in East Asian countries. In the present study, we prepared an ethyl acetate extract from the fruiting bodies of PL (PLEA) using hot water extraction, ethanol precipitation, and ethyl acetate extraction. The PLEA contained polyphenols as its major chemical component, and thus, we predicted that it may exhibit antioxidant and neuroprotective effects against oxidative stress. The results showed that the pretreatment of human brain neuroblastoma SK-N-MC cells with the PLEA (0.1-5 μg/mL) significantly and dose-dependently reduced the cytotoxicity of H2O2 and the intracellular ROS levels and enhanced the expression of HO-1 (heme oxygenase-1) and antioxidant enzymes, such as CAT (catalase), GPx-1 (glutathione peroxidase-1), and SOD-1 and -2 (superoxide dismutase-1 and -2). The PLEA also directly scavenged free radicals. PLEA pretreatment also significantly attenuated DNA fragmentation and suppressed the mRNA expression and activation of mitogen-activated protein kinases extracellular signal-regulated k...Continue Reading

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Citations

May 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wenhua ChenLingchuan Xu
Mar 22, 2019·International Journal of Molecular Medicine·Hae Jin LeeSeung Hwan Yang
Jul 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Youngin KwonYoung-Ho Lee
Feb 12, 2020·Nutrition Research and Practice·Kkot Byeol KimJung Hee Kim

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