Neuroprotective potential of the oxindole alkaloids isomitraphylline and mitraphylline in human neuroblastoma SH-SY5Y cells

3 Biotech
Mario A Tan, Seong Soo A An

Abstract

The purified oxindole alkaloids, isomitraphylline and mitraphylline from Uncaria perrottetii, revealed their ability to break amyloid aggregates in vitro suggesting their therapeutic potentials in Alzheimer's disease (AD). Thioflavin-T assay for assessing amyloid-beta (Aβ) aggregation of these alkaloids exhibited inhibitions at 60.321% ± 2.61 (50 μM) for isomitraphylline and 43.17% ± 3.48 (50 μM) for mitraphylline. Neuroprotective effects were elaborated against Aβ-induced SH-SY5Y cells at 20 μM and 10 μM for isomitraphylline, and 20 μM for mitraphylline. In addition, both alkaloids attenuated and protected the H2O2-induced SH-SY5Y cell cytotoxicity at 20 μM. The intracellular ROS levels of SH-SY5Y cells from H2O2-induced oxidative stress were reduced at 20 μM and 10 μM, and the mitochondrial membrane potentials of Aβ-induced SH-SY5Y cells were protected at 20 μM. The overall results suggested the potentials of both alkaloids to target certain pathological biomarkers of AD and could be further investigated as therapeutic or preventive drug leads against AD.

References

Jan 15, 2005·Phytochemistry·Mary E HeitzmanGerald B Hammond
Feb 14, 2007·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Teresa FrackowiakWojciech Cisowski
Mar 31, 2009·Angewandte Chemie·Roland Jakob-Roetne, Helmut Jacobsen
Jun 26, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Radosław PilarskiKrzysztof Gulewicz
Sep 24, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ta-Fu ChenR-F S Huang
Jun 14, 2014·Journal of Natural Products·Heng-Yi YuHan-Li Ruan
Jul 6, 2014·European Journal of Medicinal Chemistry·Bin YuHong-Min Liu
Aug 26, 2014·Journal of Alzheimer's Disease : JAD·Tim L EmmerzaalDeborah R Gustafson
Nov 11, 2016·Nature·J Paul TaylorDon W Cleveland
Apr 10, 2017·Biochemical Pharmacology·Paul VelanderBin Xu
Apr 23, 2017·Journal of the Neurological Sciences·Eva BagyinszkySangYun Kim
Feb 25, 2018·European Journal of Medicinal Chemistry·Devendra KumarSushil Kumar Singh
Feb 28, 2018·Frontiers in Aging Neuroscience·Devesh TewariAtanas G Atanasov
Jul 11, 2019·Journal of Natural Products·Jou-Man HuangChia-Ron Yang
Sep 13, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bruna C AzevedoMichael Wink
Nov 11, 2019·International Journal of Molecular Sciences·Cristina Angeloni, David Vauzour
Nov 20, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mario A TanSeong Soo A An
Jan 21, 2020·Journal of Agricultural and Food Chemistry·Pablo PeñalverJuan Carlos Morales
Apr 21, 2020·Neurobiology of Aging·Lianne M ReusUNKNOWN Alzheimer's Disease Neuroimaging Initiative

❮ Previous
Next ❯

Citations

Sep 21, 2021·Natural Product Research·Geane Pereira de OliveiraJacqueline Aparecida Takahashi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.