Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

ASN Neuro
Kyoko MashimaNorihiro Suzuki

Abstract

Oxidative stress plays an important role in the onset and progression of Parkinson disease. Although released dopamine at the synaptic terminal is mostly reabsorbed by dopaminergic neurons, some dopamine is presumably taken up by astroglia. This study examined the dopamine-induced astroglial protective function through the activation of the pentose-phosphate pathway (PPP) to reduce reactive oxygen species (ROS). In vitro experiments were performed using striatal neurons and cortical or striatal astroglia prepared from Sprague-Dawley rats or C57BL/6 mice. The rates of glucose phosphorylation in astroglia were evaluated using the [14C]deoxyglucose method. PPP activity was measured using [1-14C]glucose and [6-14C]glucose after acute (60 min) or chronic (15 hr) exposure to dopamine. ROS production was measured using 2',7'-dichlorodihydrofluorescein diacetate. The involvement of the Kelch-like ECH-associated protein 1 (Keap1) or nuclear factor-erythroid-2-related factor 2 (Nrf2) system was evaluated using Nrf2 gene knockout mice, immunohistochemistry, and quantitative reverse transcription polymerase chain reaction analysis for heme oxygenase-1. Acute exposure to dopamine elicited increases in astroglial glucose consumption with lac...Continue Reading

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Citations

Jul 14, 2020·Journal of Cellular and Molecular Medicine·Yi-Zheng WeiFeng Zhang
Jun 19, 2019·Biotechnology and Applied Biochemistry·Guo-Qing WangFeng Zhang
May 28, 2019·Journal of Cellular Biochemistry·Bor Luen Tang
Dec 19, 2019·Frontiers in Aging Neuroscience·Li-Yuan WangZhi-Yi He
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Sep 2, 2019·Biochemical and Biophysical Research Communications·Qiong HuangMin Xu
Mar 2, 2021·Neural Regeneration Research·Wen-Zhao WangXiao-Ling Hou
Oct 13, 2021·Annual Review of Pharmacology and Toxicology·Antonio Cuadrado

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Methods Mentioned

BETA
gene knockout
Assay
confocal microscopy
Reverse Transcription PCR
nuclear translocation

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