Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation

Stem Cells International
Shuling ZhouHasan X Avci

Abstract

Neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) are traditionally maintained and proliferated utilizing two-dimensional (2D) adherent monolayer culture systems. However, NPCs cultured using this system hardly reflect the intrinsic spatial development of brain tissue. In this study, we determined that culturing iPSC-derived NPCs as three-dimensional (3D) floating neurospheres resulted in increased expression of the neural progenitor cell (NPC) markers, PAX6 and NESTIN. Expansion of NPCs in 3D culture methods also resulted in a more homogenous PAX6 expression when compared to 2D culture methods. Furthermore, the 3D propagation method for NPCs resulted in a significant higher expression of the astrocyte markers  GFAP and aquaporin 4 (AQP4) in the differentiated cells. Thus, our 3D propagation method could constitute a useful tool to promote NPC homogeneity and also to increase the differentiation potential of iPSC towards astrocytes.

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Citations

Jul 4, 2016·Journal of Huntington's Disease·Adelaide Tousley, Kimberly B Kegel-Gleason
Oct 12, 2016·Frontiers in Cellular Neuroscience·Abinaya ChandrasekaranAndras Dinnyés
Oct 23, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J SandströmL Stoppini
Jul 8, 2018·Journal of Inherited Metabolic Disease·Sabine Jung-Klawitter, Thomas Opladen
Jun 16, 2017·Glia·Katy L H ColeDavid A Lyons
Nov 22, 2019·Aging·Constanza MorénAnthony Henry Vernon Schapira
Aug 12, 2017·Biomicrofluidics·Yachen WangJing Liu
Feb 11, 2020·Frontiers in Cellular Neuroscience·Deppo S JunejaEvangelos Delivopoulos
Jun 24, 2018·Bioengineering·Cláudia C MirandaJoaquim M S Cabral
Dec 1, 2020·Frontiers in Bioengineering and Biotechnology·Konstantinos IoannidisStavros Taraviras
Dec 17, 2020·Cells·Nicolas LeventouxHideyuki Okano
Apr 13, 2021·Frontiers in Cell and Developmental Biology·Samantha N Lanjewar, Steven A Sloan
Aug 28, 2021·International Journal of Molecular Sciences·Dmitrii A AbashkinVera E Golimbet

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Methods Mentioned

BETA
flow cytometry
PCR
FACS

Software Mentioned

AxioVision
FlowJo
OriginPro

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