Neurospheres induced from bone marrow stromal cells are multipotent for differentiation into neuron, astrocyte, and oligodendrocyte phenotypes

Biochemical and Biophysical Research Communications
Hidenori SuzukiShinya Kawai

Abstract

Bone marrow stromal cells (MSCs) can be expanded rapidly in vitro and have the potential to be differentiated into neuronal, glial and endodermal cell types. However, induction for differentiation does not always have stable result. We present a new method for efficient induction and acquisition of neural progenitors, neuronal- and glial-like cells from MSCs. We demonstrate that rat MSCs can be induced to neurospheres and most cells are positive for nestin, which is an early marker of neuronal progenitors. In addition, we had success in proliferation of these neurospheres with undifferentiated characteristics and finally we could obtain large numbers of neuronal and glial phenotypes. Many of the cells expressed beta-tubulin III when they were cultivated with our method. MSCs can become a valuable cell source as an autograft for clinical application involving regeneration of the central nervous system.

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L J RichardsP F Bartlett
Jun 1, 1993·Current Opinion in Genetics & Development·C Faust, T Magnuson
May 20, 1998·Journal of Neuroscience Research·S E Scherer, V Gallo
Jan 5, 1999·Journal of Neuroscience Methods·C N SvendsenM A Caldwell
Aug 1, 2000·Experimental Neurology·J Sanchez-RamosP R Sanberg
Oct 4, 2000·Nature Neuroscience·R GalliA L Vescovi
Feb 17, 2001·Molecular and Cellular Neurosciences·A KawaguchiH Okano
Jun 8, 2001·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·A L VescoviA Gritti
Aug 25, 2001·Biochemical and Biophysical Research Communications·Y HakamataE Kobayashi
Sep 5, 2001·Current Opinion in Genetics & Development·D Clarke, J Frisén
Sep 5, 2001·Nature Cell Biology·J G TomaF D Miller
Aug 6, 2002·Experimental Hematology·Douglas R MartinHenry J Baker

❮ Previous
Next ❯

Citations

Sep 29, 2009·Medical Molecular Morphology·Takahiro YaraToshihiko Taguchi
Apr 10, 2008·In Vitro Cellular & Developmental Biology. Animal·Hiroaki KamishinaRoger M Clemmons
Apr 11, 2008·Stem Cell Reviews·César CobaledaJesús Pérez-Losada
Sep 10, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Lamya S Shihabuddin, Seng H Cheng
May 2, 2006·Stem Cells and Development·Netta R BlondheimDaniel Offen
Feb 6, 2008·Journal of Neurosurgery. Spine·Rishi N ShethAllan D Levi
Feb 28, 2007·Clinical Laboratory·Andreas PanskyEdda Tobiasch
Oct 16, 2012·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Tim H J NijhuisSteven E R Hovius
Dec 29, 2009·Neuropharmacology·Lamya S Shihabuddin, Isabelle Aubert
Mar 24, 2007·Biochemical and Biophysical Research Communications·Na LiHuanying Zhao
Apr 3, 2008·Journal of Neuroscience Research·Ignazio BarbagalloRoberto Motterlini
Dec 14, 2011·Annals of Neurology·Luigi TitomanlioPierre Gressens
Dec 2, 2009·Cell Biology International·Purushotham Reddy KoppulaGeeta K Vemuganti
May 27, 2008·Biochemical and Biophysical Research Communications·Qin YangPeng Xie
May 5, 2005·Experimental Neurology·P Lu, M H Tuszynski
Nov 26, 2011·The International Journal of Neuroscience·Gholam Reza KakaHadi Kazemi
Apr 16, 2008·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Rodrigo SomozaFrancisco Javier Rubio
Apr 2, 2013·Experimental Neurology·Aleksandra Glavaski-Joksimovic, Martha C Bohn
Mar 28, 2013·Journal of Tissue Engineering·I-Chien LiaoHui Xu
Dec 21, 2006·Cell Transplantation·Rishi D S Nandoe TewarieMartin Oudega
Feb 24, 2015·Cell Transplantation·Hueng-Chuen FanHorng-Jyh Harn
Aug 10, 2017·Expert Opinion on Biological Therapy·Kyung-Ah Choi, Sunghoi Hong

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.