PMID: 8939449Oct 1, 1996Paper

Neurosteroid modulation of the benzodiazepine receptor in developing chick optic lobe

Neurochemistry International
L PignataroS Fiszer de Plazas

Abstract

The modulation of [3H]flunitrazepam binding by 3 alpha-hydroxy-5 alpha-dihydroprogesterone (3 alpha-OH-DHP) and 3 alpha, 5 alpha-tetrahydrodeoxicorticosterone (3 alpha-THDOC) was investigated in synaptic membranes isolated from chick optic lobe at three developmental stages, in order to evaluate the role of neurosteroids in central nervous system functional maturation. It was demonstrated that both steroids modulate [3H]flunitrazepam binding in a concentration-dependent manner at embryonic day 14, hatching and adult stage, producing maximal [3H]flunitrazepam binding enhancement at early stages of development and declining thereafter. EC50 values for 3 alpha-OH-DHP were lower than for 3 alpha-THDOC at all stages examined. On the other hand, Emax values were higher with 3 alpha-OH-DHP than with 3 alpha-THDOC. Scatchard analysis performed at embryonic day 14, hatching and adult stage showed that dissociation constants (Kd) were of 22.23 +/- 0.15, 18.38 +/- 1.15 and 19.86 +/- 0.62 nM, and maximal number of binding sites (Bmax) were 1.95 +/- 0.15, 3.13 +/- 0.21 and 2.25 +/- 0.13 pmol/mg protein, respectively. By adding 4 microM of either 3 alpha-OH-DHP or 3 alpha-THDOC, Kd values decreased significantly to 10.65 +/- 0.62, 9.71 +/- 0...Continue Reading

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Citations

Mar 5, 1999·Brain Research. Developmental Brain Research·C D BaileyJ N Reynolds
May 20, 1998·Neurochemistry International·M S ViapianoS F de Plazas
Jan 9, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M C GravielleS Fiszer de Plazas
Jun 22, 2005·Journal of Neuroscience Research·Diego J Rodríguez GilSara Fiszer de Plazas
Nov 26, 1998·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·L PignataroS F de Plazas

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