PMID: 8614258Mar 10, 1995Paper

Neurotensin stimulates neutrophil adherence to bronchial epithelial cells in vitro

Life Sciences
R A RobbinsI Rubinstein

Abstract

Neutrophils and neuropeptides have both been implicated in airway inflammation. We hypothesized that neurotensin, a neuropeptide found in the airways, would stimulate neutrophil adherence to bronchial epithelial cells. Adherence was assessed using 51Cr-labelled human neutrophils added to confluent monolayers of bovine bronchial epithelial cells. Neurotensin added to bronchial epithelial cells produced a time-and concentration-dependent increase in adherence which was maximal at 4 h and 10(-10)M (17.6 +/- 1.4% vs. 6.1 +/- 0.4%, p < 0.01). Conversely, neurotensin stimulation of neutrophils induced a concentration-dependent and rapid ( < 5 min) increase in adherence which was also maximal at 10(-10)M (27.1 +/- 1.9% vs. 10.1 +/- 1.4%, p < 0.01). The effects were reproduced by the carboxy portion of the molecule. Anti-CD11a, -CD11b or -ICAM-1 antibodies significantly decreased the neurotensin-induced increase in adherence. These data suggest an important role for neurotensin in modulating airway inflammation.

References

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Citations

Jul 24, 2012·European Journal of Pharmacology·Katarzyna Kaczyńska, Małgorzata Szereda-Przestaszewska
Oct 31, 2000·Microscopy Research and Technique·D C RubinM G Peters
Oct 17, 2019·International Journal of Molecular Sciences·Ewelina Russjan, Katarzyna Kaczyńska
Nov 30, 2019·International Journal of Molecular Sciences·Ewelina RussjanKatarzyna Kaczyńska
Apr 15, 2021·Clinical and Experimental Pharmacology & Physiology·Ewelina RussjanKatarzyna Kaczyńska

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