PMID: 9650751Jul 3, 1998Paper

Neurotoxicity evoked by N-methyl-D-aspartate in the organotypic static slice cultures of rat cerebral cortices: effect of GABA(A) receptor activation

Acta Neuropathologica
M YoshikawaY Ushio

Abstract

We investigated the neurotoxicity evoked by N-methyl-D-aspartate (NMDA) receptor stimulation in the organotypic static slice cultures of rat cerebral cortices. We also examined whether the gamma-aminobutyric acid (GABA)A receptor agonist muscimol has a protective effect on the NMDA-mediated neurotoxicity in this culture system. NMDA-mediated cytotoxicity was evaluated histologically and quantified by the measurement of lactate dehydrogenase (LDH) release into the culture medium. There was an NMDA-induced, dose-dependent leakage of LDH release and neuronal cell death, which were not attenuated by muscimol treatment. The results suggested that NMDA neurotoxicity is reproduced in the organotypic culture, and that GABA(A) receptor activation exerted no protective action against the NMDA cytotoxicity.

Citations

Nov 8, 2002·European Journal of Pharmacology·Hisashi ShirakawaAkinori Akaike
May 15, 2002·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Eugen DavidsHartmut Lüddens
Jun 26, 2009·Progress in Neurobiology·Laura LossiAdalberto Merighi
Jan 6, 2009·Journal of Pharmacological Sciences·Takahiro KatayamaMasabumi Minami
Oct 26, 2005·Journal of Neuroscience Research·Kunjan R DaveMiguel A Pérez-Pinzón

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