Neurotoxicity of mycotoxins produced in vitro by Penicillium roqueforti isolated from maize and grass silage

Human & Experimental Toxicology
Hassan MalekinejadB Ghasemsoltani-Momtaz

Abstract

Fungal growth in human foods and animal feeds causes profound damage indicating a general spoilage, nutritional losses, and the formation of mycotoxins. Thirty apparently contaminated maize and grass silage samples were analyzed for the presence of total fungi. Penicillium roqueforti were isolated from all (100%) moldy silage samples on general and selective culture media. Furthermore, P. roqueforti-positive samples culture media subjected to the toxin extraction and toxins of patulin, penicillic acid, mycophenolic acid, and roquefortin-C (ROQ-C) were identified by means of high-performance liquid chromatography method. Cytotoxicity of identified toxins was investigated on neuro-2a cells. Alamar blue reduction, neutral red uptake, and intracellular adenosine triphosphate (ATP) content assays indicated that patulin and ROQ-C exert the strongest and weakest toxicity, respectively. Reactive oxygen species (ROS) generation by the toxins-exposed cells was measured, and the results supported the mitochondrial and lysosomal dysfunction and ATP depletion in exposed cells. Our data suggest that P. roqueforti is the widely present mold in analyzed maize and grass silage samples, which is able to produce toxins that cause neurotoxicity. T...Continue Reading

References

Feb 1, 1992·Toxicology and Applied Pharmacology·R C BurghardtT D Phillips
Jun 1, 1991·Toxicology and Applied Pharmacology·R T Riley, J L Showker
Jan 1, 1982·Toxicon : Official Journal of the International Society on Toxinology·F C ChenR D Wei
Aug 12, 1999·Fungal Genetics and Biology : FG & B·J SchnürerT Börjesson
Mar 4, 2000·Journal of Food Protection·S LiD Abramson
Apr 1, 2000·Applied and Environmental Microbiology·M E BoysenJ Schnürer
Aug 5, 2000·Applied and Environmental Microbiology·I SchneweisJ Bauer
May 30, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R J Gonzalez, J B Tarloff
Jun 8, 2001·International Journal of Food Microbiology·R GeisenM Jakobsen
Nov 16, 2001·Alternatives to Laboratory Animals : ATLA·S BullS Coecke
Dec 3, 2002·Journal of the American Veterinary Medical Association·Søren R BoysenJohn E Rush
Jan 14, 2003·Journal of the American Veterinary Medical Association·Kristin L YoungMichael R Bottoff
Apr 14, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Willem G E J SchoonenEric Débiton
Jun 2, 2005·FEMS Microbiology Letters·Martin O'BrienHubert T Fuller
Nov 26, 2005·Critical Reviews in Neurobiology·Keith T LePageThomas F Murray
Mar 10, 2009·Genes & Development·Russell G Jones, Craig B Thompson
Sep 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hiromi ImamuraHiroyuki Noji

❮ Previous
Next ❯

Citations

Nov 21, 2015·Neurotoxicology·Manel BoussabbehSalwa Abid-Essefi
Sep 5, 2018·IUBMB Life·Muhammad Torequl IslamAna Amélia de Carvalho Melo-Cavalcante
Jan 22, 2020·Molecular Ecology·Emilie DumasTatiana Giraud
Aug 10, 2018·Toxicology Research·Saurabh PalKausar Mahmood Ansari
Nov 30, 2019·Mycotoxin Research·Birgitte AndersenRobert A Eustace
Apr 21, 2020·Toxicology Mechanisms and Methods·Mercedes TaroncherMaria-Jose Ruiz
Jul 28, 2020·Toxins·Sławomir GonkowskiKrystyna Makowska
Aug 20, 2021·Toxicology Reports·Vishwambar NavaleVaibhavi Dhuri

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence

Software Mentioned

Graphpad Prism

Related Concepts

Related Feeds

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Ataxia

Ataxia is a neurological condition characterized by lack of voluntary coordination of muscle movements including loss of coordination, balance, and speech. Discover the latest research on ataxia here.

Ataxias (MDS)

Ataxia is a neurological condition characterized by lack of voluntary coordination of muscle movements including loss of coordination, balance, and speech. Discover the latest research on ataxia here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.