Neurotoxicity of perineural vs intraneural-extrafascicular injection of liposomal bupivacaine in the porcine model of sciatic nerve block

Anaesthesia
M DamjanovskaT Stopar Pintaric

Abstract

Liposomal bupivacaine is a prolonged-release local anaesthetic, the neurotoxicity of which has not yet been determined. We used quantitative histomorphometric and immunohistochemical analyses to evaluate the neurotoxic effect of liposomal bupivacaine after perineural and intraneural (extrafascicular) injection of the sciatic nerve in pigs. In this double-blind prospective randomised trial, 4 ml liposomal bupivacaine 1.3% was injected either perineurally (n = 5) or intraneurally extrafascicularly (n = 5). Intraneural-extrafascicular injection of saline (n = 5) was used as a control. After emergence from anaesthesia, neurological examinations were conducted over two weeks. After harvesting the sciatic nerves, no changes in nerve fibre density or myelin width indicative of nerve injury were observed in any of the groups. Intraneural injections resulted in longer sensory blockade than perineural (p < 0.003) without persistent motor or sensory deficit. Sciatic nerve block with liposomal bupivacaine in pigs did not result in histological evidence of nerve injury.

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Citations

Nov 3, 2017·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ida ErženErika Cvetko
Aug 4, 2018·Current Opinion in Anaesthesiology·Angela Lucia BaloccoAdmir Hadzic
Apr 21, 2020·Restorative Neurology and Neuroscience·Susanna C ByramEileen M Foecking
Jan 18, 2020·Nanoscale Research Letters·Yumiao HeChao Ma
Dec 14, 2018·Frontiers in Neuroscience·Kelly A Langert, Eric M Brey
Apr 29, 2020·American Journal of Veterinary Research·Megan J McCrackenJeffrey Olivarez
Oct 25, 2020·Current Pain and Headache Reports·Alan David KayeElyse M Cornett
Mar 21, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kaixi RenJun Guo
Aug 17, 2020·Journal of Equine Veterinary Science·Stefano Leite DauRafael Fighera

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Methods Mentioned

BETA
dissection
light microscopy
PCR

Software Mentioned

ARRIVE
ViDiTo
Ellipse
LinRegPCR
Statistical Package for the Social Sciences ( SPSS

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