Neurotoxin gene profiling of clostridium botulinum types C and D native to different countries within Europe.

Applied and Environmental Microbiology
Cedric WoudstraPatrick Fach

Abstract

Clostridium botulinum types C and D, as well as their mosaic variants C-D and D-C, are associated with avian and mammalian botulism. This study reports on the development of low-density macroarrays based on the GeneDisc cycler platform (Pall-GeneDisc Technologies) applied to the simultaneous detection of the C. botulinum subtypes C, C-D, D, and D-C. The limit of detection of the PCR assays was 38 fg of total DNA, corresponding to 15 genome copies. Artificially contaminated samples of cecum showed a limit of detection below 50 spores/g. The tests were performed with a large variety of bacterial strains, including C. botulinum types C (n = 12), C-D (n = 29), D (n = 5), and D-C (n = 10), other botulinum neurotoxin (BoNT)-producing Clostridium strains (n = 20), non-BoNT-producing clostridia (n = 20), and other bacterial species (n = 23), and showed a high specificity. These PCR assays were compared to previously published real-time PCRs for the detection of C. botulinum in 292 samples collected from cases of botulism events in four European regions. The majority of the samples originated from wild birds (n = 108), poultry (n = 60), and bovines (n = 56). Among the 292 samples, 144 were positive for either the bont/C-D or the bont/D-...Continue Reading

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Citations

Nov 6, 2013·Biosecurity and Bioterrorism : Biodefense Strategy, Practice, and Science·Cédric WoudstraPatrick Fach
Nov 6, 2013·Biosecurity and Bioterrorism : Biodefense Strategy, Practice, and Science·Hanna SkarinEva Olsson Engvall
Nov 6, 2013·Biosecurity and Bioterrorism : Biodefense Strategy, Practice, and Science·Bruna AuricchioDario De Medici
Nov 6, 2013·Biosecurity and Bioterrorism : Biodefense Strategy, Practice, and Science·Fabrizio AnniballiDario De Medici
Nov 6, 2013·Biosecurity and Bioterrorism : Biodefense Strategy, Practice, and Science·Fabrizio AnniballiDario De Medici
Apr 23, 2013·Applied and Environmental Microbiology·Cedric WoudstraPatrick Fach
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Oct 3, 2015·Genome Announcements·Cédric WoudstraPatrick Fach
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May 20, 2017·Genome Announcements·Cédric WoudstraPatrick Fach
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