Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury

Stem Cells International
Xin ChenChunhua Deng

Abstract

The paracrine effect is the major mechanism of stem cell therapy. However, the details of the effect's mechanism remain unknown. The aim of this study is to investigate whether adipose tissue-derived stem cells (ADSCs) can ameliorate cavernous nerve injury-induced erectile dysfunction (CNIED) rats and to determine its mechanism. Twenty-eight days after intracavernous injection of 5-ethynyl-2-deoxyuridine- (EdU-) labeled ADSCs, the erectile function of all the rats was evaluated by intracavernosal pressure (ICP). The ADSCs steadily secreted detectable pigment epithelium-derived factor (PEDF) in vitro. The expression of PEDF increased in the penis of the bilateral cavernous nerve injury (BCNI) group for 14 days and then gradually decreased. On day 28 after the intracavernous injection, the ADSCs group exhibited a significantly increased ICP compared with the phosphate buffered saline- (PBS-) treated group. Moreover, the neuronal nitric oxide synthase (nNOS) and S100 expression in penile dorsal nerves and the smooth muscle content to collagen ratio in penile tissues significantly increased. Furthermore, elevated PEDF, p-Akt, and p-eNOS were identified in the ADSCs group. This study demonstrated that intracavernous injection of ADS...Continue Reading

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Citations

Aug 6, 2016·Arab Journal of Urology·Ezzat A Ismail, Ahmed I El-Sakka
Jan 4, 2017·Sexual Medicine Reviews·Mohammad Ayodhia SoebadiMaarten Albersen
Jul 20, 2018·Investigative Ophthalmology & Visual Science·Xiao TianQingjun Zhou
Oct 28, 2018·Nature Reviews. Urology·Uros MilenkovicFabio Castiglione
Oct 12, 2018·Expert Opinion on Biological Therapy·Serap GurWayne J G Hellstrom

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Methods Mentioned

BETA
flow cytometry
ELISA
FACS

Software Mentioned

Graph Pad
BL New Century
Pro Plus
Image
Graph Pad Prism

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