PMID: 8609594Aug 1, 1995Paper

Neurotrophic effects of the pineal gland: role of non-neuronal cells in co-cultures of the pineal gland and superior cervical ganglia

Journal of Pineal Research
J A McNultyN Tonder

Abstract

The pineal gland (PG) is a source of several trophic factors. In this study, PG and superior cervical ganglia (SCG) from Sprague-Dawley neonates (1-day-old) were co-cultured to test the hypothesis that endogenous release of PG NGF (or an NGF-like cytokine) is sufficient to promote survival of SCG neurons. Neuronal density of SCG neurons was significantly enhanced when co-cultured with PG for 7 days compared to SCG cultured alone. SCG survival and neurite formation in PG co-cultures was less than in SCG treated with exogenous NGF (100 ng/ml). The neurotrophic effect of PG co-cultures was abolished when 1% anti-NGF was added to the medium. Co-cultures of SCG neurons with established 7-day PG cultures induced extensive SCG neurite formation within 24 hr compared to SCG co-cultured with 1-day PG cultures. This suggests that PG neurotrophic effects are due to PG non-neuronal cells (nnc) that proliferate to confluency by 7 days in culture. S-antigen-positive pinealocytes did not proliferate in culture. There was decreased SCG survival when neurons were seeded onto PG cultures that had been previously killed by drying, which suggests that the neurotrophic effects of nnc are not substrate-dependent. Immunocytochemical characterization ...Continue Reading

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Citations

Mar 1, 1997·Journal of Neuroscience Research·P RedeckerS Steinlechner
Mar 1, 1996·Trends in Pharmacological Sciences·S M ReppertC Godson
Jan 22, 2002·Neuroscience·C R AndersonS M Murphy
Aug 18, 1999·Journal of Pineal Research·S Y Tsai, J A McNulty
Mar 29, 2016·Journal of Pineal Research·Haijie YuBertil Hille
Jan 8, 2015·Indian Journal of Dentistry·Abhishek Mehta, Gurkiran Kaur
Dec 5, 2014·The European Physical Journal. E, Soft Matter·Helmut R Brand, Harald Pleiner
Jul 1, 2015·The European Physical Journal. E, Soft Matter·Helmut R BrandHarald Pleiner
Nov 20, 2016·PloS One·María P Ibañez RodriguezEstela M Muñoz

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