Neurotrophin expression in neural stem cells grafted acutely to transected spinal cord of adult rats linked to functional improvement.

Cellular and Molecular Neurobiology
Ying-Li GuTing-Hua Wang

Abstract

It is well known that neural stem cells (NSC) could promote the repairment after spinal cord injury, but the underlying mechanism remains to be elucidated. This study showed that the transplantation of NSC significantly improved hindlimb locomotor functions in adult rats subjected to transection of the spinal cord. Biotin dextran amine tracing together with the stimulus experiment in motor sensory area showed that little CST regeneration existed and functional synaptic formation in the injury site. Immunocytochemistry and RT-PCR demonstrated the secretion of NGF, BDNF, and NT-3 by NSC in vitro and in vivo, respectively. However, only mRNA expression of BDNF and NT-3 but not NGF in injury segment following NSC transplantation was upregulated remarkably, while caspase-3, a crucial apoptosis gene, was downregulated simultaneously. These provided us a clue that the functional recovery was correlated with the regulation of BDNF, NT-3, and caspase-3 in spinal cord transected rats following NSC transplantation.

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Citations

Mar 11, 2015·Stem Cell Research·Ben MeadBen A Scheven
Nov 26, 2015·Acta neurochirurgica·Volker M BetzMatthias Kirsch
Dec 25, 2015·The Spine Journal : Official Journal of the North American Spine Society·Ivan ChengAlexander I Kharazi
Jun 7, 2018·Cell Transplantation·Baoyou FanShiqing Feng
Sep 11, 2013·Nihon Jibiinkoka Gakkai kaiho·Keiko HoriHideyuki Okano
Jun 10, 2016·Bulletin of Experimental Biology and Medicine·M A AleksandrovaG T Sukhikh
Jul 3, 2021·Cells·Roxana Rodríguez-BarreraAntonio Ibarra

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