Neurotrophin-induced trk receptor phosphorylation and cholinergic neuron response in primary cultures of embryonic rat brain neurons

B KnüselD R Kaplan


Tyrosine phosphorylation of trk type neurotrophin receptors in primary cultures of embryonic rat brain cells was studied by immunoprecipitation and immunoblotting. In cultures containing basal forebrain cholinergic neurons, but not in cultures of cerebral cortex, nerve growth factor (NGF) treatment for 4 min induced tyrosine phosphorylation of trk family proteins. Stimulation with brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3), resulted in a very robust phosphorylation signal in basal forebrain and cortical cultures, suggesting actions of these neurotrophins not only on cholinergic cells but probably on most embryonic brain neurons. Trk tyrosine phosphorylation was completely abolished by 5 microM K-252b. Inhibition was rapid, being evident by 30 s following addition of the drug. Corresponding stimulatory and inhibitory effects were seen for phospholipase-C gamma 1 (PLC gamma 1) and extracellular signal-regulated kinase 1 (Erk1), two enzymes involved in second messenger mechanisms. Our findings indicate involvement of trk receptor activation in the NGF response of basal forebrain cholinergic cells and provide evidence for widespread presence of BDNF and NT-3 responsive neurons in the embryonic brain.


Jul 15, 1994·Brain Research. Developmental Brain Research·H R Widmer, F Hefti
Jul 1, 1995·The Journal of Cell Biology·M D EhlersV E Koliatsos
Apr 15, 2004·Experimental Neurology·Géraldine LiotDenis Vivien
Aug 1, 2015·Molecular Neurobiology·Ana SaavedraEsther Pérez-Navarro
Sep 16, 1999·Experimental Neurology·E C Yuen, W C Mobley
Aug 26, 1998·Stroke; a Journal of Cerebral Circulation·T H LeeY Itoyama

Related Concepts

Cholinergic Fibers
Enzymes, antithrombotic
Proteins, Recombinant DNA
Nerve Growth Factor
Second messenger
Recombinant Brain-Derived Growth Factors
PC12 Cells
Brain-Derived Neurotrophic Factor Measurement

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