Neutral Competition for Drosophila Follicle and Cyst Stem Cell Niches Requires Vesicle Trafficking Genes

Genetics
Matthew S CookTodd Nystul

Abstract

The process of selecting for cellular fitness through competition plays a critical role in both development and disease. The germarium, a structure at the tip of the ovariole of a Drosophila ovary, contains two follicle stem cells (FSCs) that undergo neutral competition for the stem cell niche. Using the FSCs as a model, we performed a genetic screen through a collection of 126 mutants in essential genes on the X chromosome to identify candidates that increase or decrease competition for the FSC niche. We identified ∼55 and 6% of the mutations screened as putative FSC hypo- or hyper-competitors, respectively. We found that a large majority of mutations in vesicle trafficking genes (11 out of the 13 in the collection of mutants) are candidate hypo-competition alleles, and we confirmed the hypo-competition phenotype for four of these alleles. We also show that Sec16 and another COPII vesicle trafficking component, Sar1, are required for follicle cell differentiation. Lastly, we demonstrate that, although some components of vesicle trafficking are also required for neutral competition in the cyst stem cells of the testis, there are important tissue-specific differences. Our results demonstrate a critical role for vesicle trafficki...Continue Reading

References

Nov 1, 1979·Journal of Ultrastructure Research·R W HardyM Garavito
Nov 1, 1996·The Journal of Cell Biology·J H Koenig, K Ikeda
Aug 12, 1998·The Journal of Cell Biology·M N SeamanS D Emr
Nov 14, 1998·Seminars in Cell & Developmental Biology·M T Fuller
Oct 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Xiaoqing Song, Ting Xie
Jun 5, 2003·Developmental Biology·James J FabrizioStephen DiNardo
Oct 28, 2003·Nature Cell Biology·Michelle A LiDorothea Godt
Nov 19, 2003·Mechanisms of Development·Marcos González-Gaitán
Jun 15, 2007·Nature Cell Biology·Matthias BehrMichael Hoch
Jul 17, 2007·Current Biology : CB·Angshuman SarkarCordula Schulz
Mar 29, 2008·Cell Stem Cell·Todd Nystul, Allan Spradling
Jul 11, 2008·Molecular Biology of the Cell·Viorica IvanCatherine Rabouille
Aug 23, 2008·Nature Reviews. Molecular Cell Biology·Sander van den Heuvel, Nicholas J Dyson
Jun 30, 2009·Current Opinion in Genetics & Development·Frederik Wirtz-Peitz, Jennifer A Zallen
Dec 8, 2009·Developmental Biology·Andre Franz, Veit Riechmann
Jan 5, 2010·Current Biology : CB·Dominique FörsterStefan Luschnig
Nov 26, 2010·The Journal of Cell Biology·Tiffiney R HartmanAlana M O'Reilly
Apr 5, 2011·Nature Methods·Jian-Quan NiNorbert Perrimon
Apr 15, 2011·Molecular Biology of the Cell·Jason BurgessJulie A Brill
Jul 14, 2011·Development·Allon M Klein, Benjamin D Simons
Jun 8, 2012·Molecular Biology of the Cell·Tomohiro Yorimitsu, Ken Sato
Oct 20, 2012·Developmental Cell·Cynthia ViedDaniel Kalderon
Feb 5, 2013·Cold Spring Harbor Perspectives in Biology·Christopher LordElizabeth A Miller
Mar 20, 2013·The Journal of Cell Biology·Ke ZhangHugo J Bellen
Jun 27, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·Pankaj Sahai-HernandezTodd G Nystul
Nov 23, 2013·Science·Louis VermeulenDouglas J Winton
Sep 27, 2014·Genome Research·Nele A HaeltermanHugo J Bellen
Oct 10, 2014·American Journal of Physiology. Renal Physiology·Ben FogelgrenJoshua H Lipschutz
Sep 12, 2015·Annual Review of Cell and Developmental Biology·Leah Joy GreenspanErika Matunis

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