PMID: 7538997Feb 1, 1995Paper

Neutral endopeptidase modulates substance P-induced vasodilation in vivo

Journal of Applied Physiology
X P Gao, I Rubinstein

Abstract

The purpose of this study was to investigate whether neutral endopeptidase (NEP; EC 3.4.24.11) modulates substance P-induced vasodilation in the oral mucosa in vivo. Using intravital microscopy, we measured the diameter of second-order arterioles (44-70 microns) in the hamster cheek pouch during suffusion of capsaicin and substance P. We found that capsaicin (0.1 and 10.0 nM) induced significant concentration-dependent vasodilations (13 +/- 4 and 39 +/- 7% increase from baseline, respectively; P < 0.05) that were significantly potentiated by phosphoramidon (10.0 nM), a selective NEP inhibitor (35 +/- 15 and 61 +/- 12% increase from baseline, respectively; P < 0.05). Substance P (0.1 and 10.0 nM) also induced significant concentration-dependent vasodilations (7 +/- 3 and 25 +/- 8% increase from baseline, respectively; P < 0.05) that were mediated by the COOH-terminal of the molecule. Substance P-induced responses were significantly potentiated by phosphoramidon (34 +/- 9 and 53 +/- 10% increase from baseline, respectively; P < 0.05) and thiorphan (10.0 microM), a selective NEP inhibitor (44 +/- 11 and 53 +/- 10% increase from baseline, respectively; P < 0.05). Substance P-(1-9) had no significant effects on arteriolar diameter. ...Continue Reading

Citations

Jul 2, 2015·Current Allergy and Asthma Reports·Jonathan A Bernstein, Umesh Singh
Jun 12, 2009·Journal of Hypertension·Robert M K W LeeYu-Jing Gao
Oct 31, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Michelle L SpennyNicole S Gibran
Jun 1, 1997·The Annals of Otology, Rhinology, and Laryngology·M ZakkarI Rubinstein

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