PMID: 6405073Feb 1, 1983Paper

Neutral proteinase activity produced in vitro by cells of the glomerular mesangium

Kidney International
D H LovettJ L Ryan

Abstract

Cells derived from isolated glomerular tufts of rats were studied in primary tissue culture after the removal of epithelial cells by collagenase treatment. The cultured cells, fusiform or stellate in shape, grew readily over a 12-day period. Immunofluorescence staining was positive for myosin and fibronectin, while negative for Factor VIII, suggesting that the outgrowing cells were derived from the glomerular mesangium. In serum-free culture, these cells produced neutral proteinase activity that occurred as a latent trypsin-activable form (apparent molecular weight range, 78,000 to 100,000 daltons) and in an active form (44,000 to 58,000 daltons). Neutral proteinase activity was inhibited by EDTA and by cysteine, and exhibited a pH optimum of 7.2 to 7.8, characteristic of an extracellularly active metalloendopeptidase. The culture supernate which contained the neutral proteinase activity was capable of degrading purified rat glomerular basement membrane. The release of hydroxyproline-containing fragments from the basement membrane indicated that degradation of the type IV collagen component of the basement membrane was occurring. These findings suggest that the neutral proteinase activity generated by mesangium-derived cells ma...Continue Reading

References

Aug 1, 1978·The Journal of Experimental Medicine·J Schnyder, M Baggiolini
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·Y S Kanwar, M G Farquhar
Jan 1, 1976·Annals of the New York Academy of Sciences·S Gordon
May 1, 1977·The Biochemical Journal·A SellersJ J Reynolds
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·L A LiottaG R Martin
Jun 1, 1979·European Journal of Immunology·W R McMaster, A F Williams
Jan 1, 1979·Kidney International·J T HjelleE Meezan
Jan 1, 1979·International Review of Cytology·N A KefalidesC C Clark
Jul 1, 1978·Kidney International·J I KreisbergM J Karnovsky
Jul 1, 1973·The Journal of Pathology·F Walker
May 1, 1967·Analytical Biochemistry·K I KivirikkoD J Prockop
Jun 1, 1982·European Journal of Clinical Investigation·C H DuboisP R Mahieu
Feb 1, 1980·Kidney International·A F MichaelS M Mauer
Jan 29, 1980·Biochemical and Biophysical Research Communications·M P Cohen
Apr 1, 1980·Kidney International·R C AtkinsW W Hancock
Dec 1, 1960·Journal of Ultrastructure Research·H LATTAS C MADDEN

❮ Previous
Next ❯

Citations

Apr 1, 1991·Transplant International : Official Journal of the European Society for Organ Transplantation·U HempelmannH Tschesche
Jan 1, 1995·Cancer Chemotherapy and Pharmacology·M Goppelt-Struebe, I Winter
Sep 15, 1982·Klinische Wochenschrift·R B SterzelM Kashgarian
May 1, 1994·Atherosclerosis·E F GröneD Seidel
Sep 30, 1988·Biochemical and Biophysical Research Communications·W H BaricosS V Shah
Sep 1, 1996·The Journal of Laboratory and Clinical Medicine·R SharmaV J Savin
Dec 20, 2002·International Immunopharmacology·Julie Christopher, Ayad A Jaffa
Jun 6, 2003·Kidney International·Beate BieritzAndrea Hartner
Aug 22, 2000·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·N GaglianoG Annoni
Oct 2, 2004·Kidney International·Anja S MühlfeldCharles E Alpers
Sep 17, 2005·Kidney International·John Keeling, Guillermo A Herrera
Aug 1, 1985·The Journal of Clinical Investigation·E P MacCarthyB S Ooi
Sep 1, 1987·The Journal of Clinical Investigation·H E AbboudP DiCorleto
Jul 1, 1988·The Journal of Clinical Investigation·D H Lovett, A Larsen
Jan 1, 1991·The Journal of Clinical Investigation·D RitterJ E Greenwald
Jul 15, 1996·The Journal of Clinical Investigation·B S VishwanathB M Frey
Mar 31, 2009·Journal of the American Society of Nephrology : JASN·Soraya BengattaBrigitte Lelongt
Jan 20, 2000·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·K SebekováA Heidland
Feb 9, 2008·Regulatory Peptides·Atsuo TaharaMasayuki Shibasaki
Jun 1, 1988·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·W G Couser
Aug 1, 1992·Kidney International·V MontinaroA Rifai
May 1, 1988·Kidney International·S M Bonsib
Aug 1, 1991·Kidney International·W H Baricos, S V Shah
Oct 1, 1986·Kidney International·M S Simonson, M J Dunn
Aug 1, 1986·Kidney International·D H Lovett, R B Sterzel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.