PMID: 7523700Nov 1, 1994Paper

Neutralization of bovine papillomavirus by antibodies to L1 and L2 capsid proteins

Journal of Virology
R B RodenJ T Schiller

Abstract

We have generated four mouse monoclonal antibodies (MAbs) to bovine papillomavirus virions that bound type-specific, adjacent, and conformationally dependent epitopes on the L1 major capsid protein. All four MAbs were neutralizing at ratios of 1 MAb molecule per 5 to 25 L1 molecules, but only three effectively blocked binding of the virus to the cell surface. Therefore, antibodies can prevent papillomavirus infection by at least two mechanisms: inhibition of cell surface receptor binding and a subsequent step in the infectious pathway. The neutralizing epitopes of the bovine papillomavirus L2 minor capsid protein were mapped to the N-terminal half of L2 by blocking the neutralizing activity of full-length L2 antiserum with bacterially expressed peptides of L2. In addition, rabbit antiserum raised against amino acids 45 to 173 of L2 had a neutralizing titer of 1,000, confirming that at least part of the N terminus of L2 is exposed on the virion surface.

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Citations

Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·B ChackerianJ T Schiller
Apr 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·P LenzJ T Schiller
Dec 25, 2003·Journal of Virology·Christopher B BuckJohn T Schiller
May 14, 2005·Journal of Virology·Ioannis BossisPatricio I Meneses
Sep 23, 2006·Nature Reviews. Cancer·Richard Roden, T-C Wu
Aug 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eduardo ZamoraReinhard Kirnbauer
Feb 1, 2003·The Journal of Biological Chemistry·Rongcun YangRichard B S Roden
Mar 1, 2003·Journal of Virology·Rongcun YangRichard B S Roden
Aug 20, 2002·The Journal of General Virology·P J Klasse, Q J Sattentau
May 4, 2007·Journal of Virology·Valerie LanioszPatricio I Meneses
May 15, 2007·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·M Müller, L Gissmann
May 28, 2009·Journal of the National Cancer Institute·Subhashini JaguRichard B S Roden
Mar 29, 2013·Journal of Virology·Subhashini JaguRichard B S Roden
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·H L GreenstoneJ T Schiller
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·H NaoraR B Roden
Jul 10, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·John K Chan, Jonathan S Berek
Nov 21, 2008·Journal of Virology·Mohammed Rafii-El-Idrissi BenhniaShane Crotty
Oct 12, 2007·Journal of Virology·Ratish GambhiraRichard B S Roden
May 8, 2009·Immunology and Cell Biology·Balasubramanyam KaranamRichard B S Roden
May 16, 2008·Veterinary Research·Giuseppe Borzacchiello, Franco Roperto
Feb 2, 2010·Expert Reviews in Molecular Medicine·Allison H Bartlett, Pyong Woo Park
Mar 29, 2013·PloS One·Kihyuck KwakRichard B S Roden
Oct 28, 2016·Microbiology and Molecular Biology Reviews : MMBR·Laura A VanBlarganTheodore C Pierson
Mar 11, 2005·Expert Opinion on Emerging Drugs·Neil D Christensen
Dec 3, 2003·Journal of Virology·Saeed Shafti-KeramatReinhard Kirnbauer
Mar 3, 2018·Nature Reviews. Cancer·Richard B S Roden, Peter L Stern

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