PMID: 6171240Jan 1, 1981Paper

Neutralization of different strains of murine cytomegalovirus (MCMV)-effect of in vitro passage

Archives of Virology
K T ChongC A Mims

Abstract

Two strains of mouse cytomegalovirus (MCMV) propagated in mouse salivary glands could be distinguished in neutralization tests. Cell (mouse embryo fibroblast)-passaged virus of either strain was generally 10-20 times easier to neutralize than virus produced in mouse salivary glands. Evidence was obtained that mouse immunoglobulin was attached to virus particles in salivary gland preparations. First, antibody to mouse immunoglobulin inactivated salivary gland virus but not cell culture-grown virus. Second, virus obtained from mice early after infection, before antibody production, was as easy to neutralize as cell culture-grown virus. The attached immunoglobulins were CMV-specific, non-neutralizing, and interfered with the action of neutralizing antibody. Non-neutralizing antibodies that bind to infectious virus particles are present in mouse serum at the time salivary glands are harvested, 3 weeks after infection.

References

Jul 1, 1976·Virology·J B HudsonT R Mosmann
Aug 1, 1977·The Journal of General Virology·P Talbot, J D Almeida
Jan 1, 1979·Microbiology and Immunology·Y Eizuru, Y Minamishima
Dec 1, 1981·The Journal of General Virology·K T Chong, C A Mims
Feb 1, 1971·Infection and Immunity·J E Osborn, D L Walker

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