PMID: 9557629Apr 29, 1998Paper

Neutralization of human immunodeficiency virus type 1 by antibody to gp120 is determined primarily by occupancy of sites on the virion irrespective of epitope specificity

Journal of Virology
P W ParrenQ J Sattentau

Abstract

We investigated the relative importance of binding site occupancy and epitope specificity in antibody neutralization of human immunodeficiency virus (HIV) type 1 (HIV-1). The neutralization of a T-cell-line-adapted HIV-1 isolate (MN) was analyzed with a number of monovalent recombinant Fab fragments (Fabs) and monoclonal antibodies with a range of specificities covering all confirmed gp120-specific neutralization epitopes. Binding of Fabs to recombinant monomeric gp120 was determined by surface plasmon resonance, and binding of Fabs and whole antibodies to functional oligomeric gp120 was determined by indirect immunofluorescence and flow cytometry on HIV-infected cells. An excellent correlation between neutralization and oligomeric gp120 binding was observed, and a lack of correlation with monomeric gp120 binding was confirmed. A similar degree of correlation was observed between oligomeric gp120 binding and neutralization with a T-cell-line-adapted HIV-1 molecular clone (Hx10). The ratios of oligomer binding/neutralization titer fell, in general, within a relatively narrow range for antibodies to different neutralization epitopes. These results suggest that the occupancy of binding sites on HIV-1 virions is the major factor in...Continue Reading

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Citations

Jan 31, 2003·AIDS·Elaine R ThomasAine McKnight
Apr 12, 2003·AIDS Research and Human Retroviruses·Zhiwei WuDaniel Malamud
Feb 24, 2005·AIDS Research and Human Retroviruses·Oliver HartleyJohn P Moore
Apr 20, 2017·Current Opinion in HIV and AIDS·Max Medina-RamírezQuentin J Sattentau
Mar 13, 2001·Annual Review of Immunology·P PoignardD R Burton
Apr 11, 2008·European Biophysics Journal : EBJ·Thomas EndressDon C Lamb
Mar 18, 2004·Reviews in Medical Virology·Nigel J Dimmock, Sam A Hardy
May 23, 2007·Archives of Virology·S A Reading, N J Dimmock
Mar 23, 2006·Annual Review of Immunology·Ralph Pantophlet, Dennis R Burton
Apr 18, 2009·Current Opinion in HIV and AIDS·Quentin Sattentau
Jun 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Dennis R BurtonJohn P Moore

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