Neutralization of human papillomavirus by specific nanobodies against major capsid protein L1

Journal of Microbiology and Biotechnology
Sara MinaeianOliver Jay Broom

Abstract

The human papillomavirus (HPV) is the main cause of cervical cancer in developing countries. Rapid diagnosis and initiation of treatment of the HPV infection are critical. Various methods have been employed to reduce the immunogenicity of antibodies targeting HPV serotypes. Nanobodies are the smallest fragments of naturally occurring single-domain antibodies with their antigenbinding site compromised into a single domain. Nanobodies have remarkable properties such as high stability, solubility, and high homology to the human VH3 domain. In this study, a phagemid library was employed to enrich for nanobodies against the L1 protein of the human papilloma virus. Binding reactivity of the selected clones was evaluated using phage enzyme-linked immunosorbent assay (phage-ELISA). Finally, two nanobodies (sm5 and sm8) with the best reactivity against the Gardasil vaccine and the purified HPV-16 L1 protein were expressed and purified using a Ni(+)-NTA column. The accuracy of expression and purification of the nanobodies was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting assays. In vitro studies demonstrated that neutralization was achieved by the selected nanobodies. The ease of gen...Continue Reading

Citations

Sep 8, 2012·ISRN Surgery·P W J van RutteS W Nienhuijs
Oct 19, 2017·Diagnostics·Chiuan Herng LeowJames McCarthy
Dec 21, 2019·Immunological Investigations·Marzieh SanaeiMohammad Hossein Yazdi
Mar 7, 2019·Frontiers in Genetics·Amanat AliRanjit Vijayan
Mar 1, 2020·Drug Discovery Today·Yunfeng LinLei Zhang

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